lecture 26 - MCB 354 Lecture 4 Glycolysis, Part II...

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MCB 354 Lecture 4 Glycolysis, Part II Fermentation Glycogen Reading in Lehninger : Chapter 14
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STEP #7: ENERGY TRANSFER TO ATP SUBSTRATE LEVEL PHOSPHORYLATION PHOSPHOGLYCERATE KINASE OVERALL THE ENERGY RELEASED IN THE OXIDATION OF AN ALDEHYDE TO A CARBOXYLATE IS CONSERVED THROUGH THE FORMATION OF ATP 4-1
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STEP #8: SHIFT OF A SUBSTITUENT GROUP: MUTASES 4-2
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STEP #8 PHOSPHO- GLYCERATE MUTASE 4-3
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STEP #9: DEHYDRATION TO FORM PHOSPHOENOL PYRUVATE - ENOLASE WOULD TAKE A VERY STRONG BASE TO PULL OFF THE C-2 PROTON pKa = 32 4-4
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ENOLASE ACTIVE SITE NOTE TWO Mg IONS PROVIDE ELECTROSTATIC STABILIZATION AND MAKE THE C-2 CARBON MORE ACIDIC - EASIER TO DEPROTONATE STEP #9: ENOLASE 4-5
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ENOLASE REACTION MECHANISM NOTE HOW METAL COORDINATION CAN DRAMATICALLY AFFECT THE INHERENT “ELECTRON AFFINITY” STEP #9: ENOLASE 4-6
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STEP #10: ATP GENERATION FROM PEP PYRUVATE KINASE 4-7
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TAUTOMERIZATION ADDS A LARGE NEGATIVE
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This note was uploaded on 01/29/2012 for the course MCB 354 taught by Professor Spees during the Spring '09 term at University of Illinois, Urbana Champaign.

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lecture 26 - MCB 354 Lecture 4 Glycolysis, Part II...

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