topic 7_rivier_252 - MCB 252 Cell Biology Intermediate...

Info iconThis preview shows pages 1–16. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: MCB 252 Cell Biology Intermediate filaments (IFs) Reading = Lodish section 18.7 Genetic Analysis Lodish pp 166-167 MCB 252, Part 2 Topic 7 Cytoskeleton: Intermediate Filaments MCB 252, Part 2 Topic 7 Cytoskeleton: Intermediate Filaments Intermediate filaments (IFs) Classes Structure of monomer/polymer Dynamics Functions Intermediate filament network in epithelial cells Alberts, et al., Fig. 16-18 Keratin ; Cell boundaries IFs are extremely stable Pollard and Earnshaw, Fig. 35-4 Cell extracted with detergent, high salt, stained for IF L V PAGE Cytoplasmic vs. Nuclear IFs Vertebrates have over 50 genes encoding 6 classes of IF proteins Differentially expressed in nearly all cells of the body desmin- muscle GFAP- glia NFs- neurons keratins- epithelial cells Generally constitute ~1% of cellular proteins; in some cell types (e.g., neurons and epidermal keratinocytes) can be ~85% of total protein Specificity of IF expression Intermediate filament structure Pollard and Earnshaw, Fig. 35-2 Evolution of IF Superfamily Lamins were the progenitors Cytoplasmic IFs arose in Animals after the branching off from plants Lamins lost NLS and Prenylation region (membrane attachment) resulting in cytoplasmic localization Cytoplasmic Lamins formed several families each with specific functions: skin, muscles, nerves alpha-helical coiled coil Alberts, et al., Fig. 3-11 Lodish, Fig. 3-6c How can one determine the orientation of monomers in dimers and tetramers? If N- and C-termini are quite different in size: Lodish, Fig. 19-33 How can one determine the orientation of monomers in dimers and tetramers? If N- and C-termini are similar in size? (Lodish, Fig. 5-51) immunogold electron microscopy dimer or? tetramer tetramer Polymer structure Alberts, et al., Fig. 16-16 Polymer structure Pollard and Earnshaw, Fig. 35-3 Lodish, Fig. 18-45 IF Dynamics Fluorescence recovery after photobleaching (FRAP) Photobleach GFP-vimentin: does it recover?...
View Full Document

Page1 / 44

topic 7_rivier_252 - MCB 252 Cell Biology Intermediate...

This preview shows document pages 1 - 16. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online