topic 12_rivier_252 - MCB 252 Topic 12 Cell Adhesion Lodish...

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MCB 252 Topic 12 Cell Adhesion Lodish 19.0 - 19.2
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MCB 252, Part 2 Cell-cell/ECM interactions, Cell-cell adhesion Background (experimental embryology) Cell adhesion molecules Cadherins Identification Functional analysis Cadherin-associated proteins
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Early experiments to understand cell adhesion H.V. Wilson (1907)- sponge cell adhesion http://www.mbl.edu/animals/images/microciona.3.jpg pass through sieve (mechanical dissociation) individual cells (not to scale) reaggregate
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pass through sieve (mechanical dissociation) mix ? Use two different species:
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pass through sieve (mechanical dissociation) mix ? Use two different species: sort sort
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Embryogenesis Red = ectoderm Blue = Neural Green = mesoderm Yellow = endoderm Alberts 22-75
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Reaggregation of amphibian embryonic cells (Townes and Holtfreter, 1955) http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=dbio.figgrp.379
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Sorting in amphibian embryonic cell aggregates http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=dbio.figgrp.380 Epidermis + Mesoderm Mesoderm + Endoderm Neural plate + Epidermis Neural plate + Mesoderm + Epidermis Epidermis + Mesoderm + Endoderm
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Cell sorting in embryonic organs http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=dbio.figgrp.384 1. Dissociate embryonic organs 2. Combine 3. Cells reaggregate 4. Sort according to organ type 5. Specific patterns of aggregation Differential adhesion hypothesis (Steinberg): Cells sort due to differences in adhesive properties (could be quantitative or qualitative)
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Three ways in which surface molecules can mediate adhesion Alberts, Fig. 19-26 Aggregation experiments defined two types of adhesions: Ca 2+ -dependent and Ca 2+ -independent
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How can one identify adhesion molecules? First, need an assay: in vitro aggregation. dissociated cells in Petri dish rotate slowly for 1 hour aggregate Where are the molecules that mediate adhesion localized?
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