topic 14_rivier_252 - MCB 252 Cell Biology Topic 14 Cell...

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Unformatted text preview: MCB 252 Cell Biology Topic 14 Cell Cycle: Genetic Analysis of the Yeast Cell Cycle MCB 252, Cell cycle I Embryonic cell cycle & Biochemical analysis Basic Features Cell fusion experiments Embryonic cell cycles Xenopus (biochemical analysis) Identification of MPF Identification of Cyclins The Eukaryotic Cell Cycle Alberts, et al. Fig 17- 13 Flow cytometry to analyze DNA content fluorescence- activated cell sorter (FACS) Lodish, et al. Fig. 9-28 Label cells with a DNA dye Cell fusion experiments Fuse cells together, monitor effects S G1 + G1 nucleus enters S S G2 + G2 nucleus does not enter S S phase cells have factor that can induce DNA replication in G1 cells G2 cells do not respond to this factor. Block to re- replication: replicate once and only once Fuse mitotic cells w/ interphase cells In all cases, the nuclear envelope breaks down and chromosomes condense. Pollard and Earnshaw, Fig. 46-4; see Lodish Fig....
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topic 14_rivier_252 - MCB 252 Cell Biology Topic 14 Cell...

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