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Unformatted text preview: are many nitrogens and carbonyl groups which contribute to its polar status. 6. If the stationary phase was changed to be nonpolar and the mobile phase was now polar, then the substances that were more polar would actually travel farthest in this situation. The distances would be opposite of what was seen in the experiment, similar to flipping the TLC plate over and then measuring distances. 7a. A nonpolar mobile phase should be used instead of the polar 1% acetic acid in ethyl acetate. This would make the unknown, which is most likely nonpolar based on its previous Rf value, travel a short distance, which would then be an Rf of .25. 7b. Petroleum ether is an example of a nonpolar mobile phase that would provide the desired Rf value....
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This note was uploaded on 01/29/2012 for the course CHEM 233 taught by Professor Staff during the Spring '08 term at University of Illinois, Urbana Champaign.
- Spring '08