5 Microbial Growth 1-28-11 - Bacterial Growth Bacterial...

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Bacterial Growth Bacterial Growth Most bacteria reproduce by binary fission 1 2 4 8 16 32 Called exponential growth Graph in a number of different ways etc
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Plot # cells on a linear scale Plot # cells on a linear scale Time # cells 64 128 256 512 1024
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Bacterial Growth Bacterial Growth # cells log (# cells) 1 0.000 2 0.301 4 0.602 8 0.903 16 1.204 32 1.505 64 1.806 128 2.107 256 2.408 512 2.709 1024 3.010 log b x = n b n = x log of 1 = 0 10 0 = 1 log of 10 = 1 10 1 = 10 log of 100 = 2 10 2 = 100 log of 1,000,000 = 6 10 6 = 1,000,000 log of 2 = 0.301 10 0.301 = 2
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Plot log (# cells) on a linear scale Plot log (# cells) on a linear scale Time log (# cells) 0 1.0 2.0 3.0 1.806 2.107 2.408 2.709 3.010 1.505 1.204 0.903 0.602 0.301 0
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Plot # cells on a log scale Plot # cells on a log scale Time # cells 64 128 256 512 1024 32 16 8 4 2 1
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Plot # cells on a log scale Plot # cells on a log scale Time 64 128 256 512 1024 32 16 8 4 2 1 The steeper the slope the faster the growth (e.g., doubling time is shorter) Doubling time is the time it takes for the number of cells to double in number # cells
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The Growth Curve The Growth Curve Inoculate bacterial cells into a liquid broth and incubate Called a batch culture if it’s a closed system (no additional media added, nutrients decrease and waste products increase) Plot # of cells with time Bacterial cells undergo different stages of growth Lag phase Log phase (or logarithmic phase or exponential phase) Stationary phase Death phase Figure 4.20
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The Growth Curve 1) Lag phase
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This note was uploaded on 01/29/2012 for the course MCB 300 taught by Professor Salyers during the Spring '08 term at University of Illinois, Urbana Champaign.

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5 Microbial Growth 1-28-11 - Bacterial Growth Bacterial...

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