011 Antibodies as Tools and GI Tract Infections 4-13-11 NEW SECTION

011 Antibodies as Tools and GI Tract Infections 4-13-11 NEW SECTION

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Antibodies as Tools Antibodies as Tools
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Blood Group Systems Blood Group Systems
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ABO Blood Group Antigens ABO Blood Group Antigens A type B type AB type O type
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Antibody Anti - A Anti - B Blood Type A B O Typing Blood: Typing Blood: An Example of An Example of Agglutination Agglutination AB
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ABO Blood Types in the US ABO Blood Types in the US O 45-49% A 27-40% B 11-20% AB 4%
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Type Bacteria by Agglutination Type Bacteria by Agglutination E. coli and Salmonella O antigens = LPS H antigens = flagella Example: E. coli O157:H7
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Viral Infection is Usually Viral Infection is Usually Detected by Assaying the Detected by Assaying the Antibody Response Antibody Response Example: HIV
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ELISA ELISA E nzyme - L inked I mmuno S orbent A ssay
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Microtiter Plate Microtiter Plate
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ELISA to Detect Antibody ELISA to Detect Antibody Antigen Sample (specific 1° Antibody, if present) 2° Antibody Enzyme Linked Detection Substrate See also Figure 28.12
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Western Blot Western Blot Separate Proteins on Polyacrylamide Gel Transfer to Paper Probe with Ab Detect with Labeled 2 ° Antibody Y *
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ELISA to Detect Antigen ELISA to Detect Antigen 1° Antibody Sample 2° Antibody Enzyme Linked Detection Substrate (specific Ag, if present) See also Figure 28.13
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Myeloma Y Y Y Y B Cells from Spleen Immunized Mouse Y Hybridoma Y Y Monoclonal Antibody Polyclonal Antibodies vs Polyclonal Antibodies vs Monoclonal Antibodies Monoclonal Antibodies Y Fusion
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Fluorescent Ab Against Fluorescent Ab Against E. coli E. coli O157 O157
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Total Cells = 1,257 Positive Cells = 375 Flow Cytometry Flow Cytometry Fluorescence Activated Fluorescence Activated Cell Sorter (FACS) Cell Sorter (FACS)
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Y YYYYYYY YYYYYYY Yes No Pregnancy Test Pregnancy Test YYYYYYY Anti - Antibody Control YYYYYYY Anti - Hormone Test Human Gonadotropin
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Home Pregnancy Tests Home Pregnancy Tests
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YYYYYYY Control Line Anti-Antibody Test Line Anti - Hormone YYYYYYY YYYYYYY YYYYYYY Y Dye–Labelled Anti–Hormone Pregnant Not Pregnant
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Gastrointestinal Pathogens Gastrointestinal Pathogens Water and Food - Borne Disease ~76 million each year in US 300,000 hospitalized 5,000 deaths
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Organism Illnesses Hospitalizations Deaths Norwalk-like viruses 23,000,000 50,000 310 Rotavirus 3,900,000 50,000 30 Astrovirus 3,900,000 12,500 10 Campylobacter spp 2,453,926 13,174 124 Giardia lamblia 2,000,000 5,000 10 Salmonella, nontyphoidal 1,412,498 16,430 582 Shigella spp 448,240
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This note was uploaded on 01/29/2012 for the course MCB 300 taught by Professor Salyers during the Spring '08 term at University of Illinois, Urbana Champaign.

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011 Antibodies as Tools and GI Tract Infections 4-13-11 NEW SECTION

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