Whitaker 2 2-25-11 - Whitaker Office Hours Wednesdays...

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Whitaker Office Hours Wednesdays 2:00-3:00 Fridays 3:00-4:00 CLSL C222
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It is a microbial world
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Outline Metabolic diversity (lectures 1-3) Biology is chemistry. The search for free energy Microbial Metabolism and Geochemical cycles Carbon cycle Nitrogen cycle Microbial Metabolism and Environmental health Sulfur Phosphorous Iron and other metals
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Ethanol Fermentation In what type of environment will you find fermentation? Anaerobic
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Diversity of (delicious) fermentation end products
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Anaerobic Respiration Microbial organotrophs can use other electron acceptors like Nitrate and Sulfate if Oxygen is not present Does anaerobic respiration yield more or less energy than aerobic respiration?
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Microbial organotrophs Many microbes eat sugars and breathe oxygen just like you do in aerobic respiration. Organotrophic microbes can also metabolize a huge diversity of other organic substrates Fermentation is another form of anaerobic organotrophy that uses another organic substrate as the electron acceptor. Microbial organotrophs can also use alternative electron acceptors like SO 4 -2 or NO 3 - in anaerobic respiration.
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Phototrophy Energy from light H 2 + 1/2 O 2 H 2 O Full 237.34 kJ/mole
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Donor ? Acceptor ? Photosynthesis
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Donor - H 2 /H 2 0, Acceptor NADP/NADPH Photosynthesis
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Lower wavelength = higher energy PSII PSI
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Photosystem II P680 enough energy to oxidize H 2 O
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QuickTime™ and a YUV420 codec decompressor are needed to see this picture. http://svs.gsfc.nasa.gov/search/Instrument/SeaWiFS.html
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Chloroplasts (microbes too)
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Chloroplasts are ancient endosymbiosis of cyanobacteria
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Anaerobic photosynthesis Photosystem I
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Anaerobic photosynthesis Photosystem I peak absorbtion at 840nm (infrared)
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Anaerobic photosynthesis Photosystem I peak absorbtion at 840nm (infrared)
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Chlorobium Instead of H 2 O to O 2 as we are used to. Photosyntesis H 2 S to S 0 Green Sulfur Bacteria Chlorobium aggregatum Chlorobium
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Purple Sulfur Bacteria Chromatium
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Phototrophs build stratified communities based on light absorbance Microbial Mat
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Anaerobic photosynthesis Photosystem II
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Anaerobic photosynthesis Photosystem I peak absorbtion at 870nm (infrared)
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Anaerobic photosynthesis Photosystem II
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Rhodospirillum rubrum Purple non-sulfur
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Bacteriorhodopsin Retinal pumps protons out of the membrane Cells harvest the proton motive force
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This note was uploaded on 01/29/2012 for the course MCB 300 taught by Professor Salyers during the Spring '08 term at University of Illinois, Urbana Champaign.

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Whitaker 2 2-25-11 - Whitaker Office Hours Wednesdays...

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