Ex 08 Lab Pract Prep Sht KEY

Ex 08 Lab Pract Prep Sht KEY - KEY: Bio 205L EXERCISE 8...

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KEY: Bio 205L EXERCISE 8 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). Section 8.1: Loading Protein samples into wells of a Polyacrylamide Gel a. Heat protein samples to 60 o C in a water bath. b. Know how to properly operate a microcentrifuge. c. Check wells of the gel to ensure they are in good condition. d. Attach the gel sandwich to the electrophoresis apparatus. e. Know how to forcefully wash the wells of the gel before loading samples. f. Know how to load samples directly into the wells of a gel without spillover into other lanes. g. Fill wells with Tank Buffer (electrophoresis running buffer). h. Know how to place gel in electrophoresis chamber and attach to power supply. Section 8.2: Electrophoretic Separation of Proteins a. Multiply the desired milliAMPs by the number of gels that will be attached to the power supply. b. Know the four ways to check and see if the gel is running properly.
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This note was uploaded on 01/30/2012 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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Ex 08 Lab Pract Prep Sht KEY - KEY: Bio 205L EXERCISE 8...

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