Ex02_Analysis_Questions - Group Members: _Exercise_Date: _...

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Group Members: _________________________________________________Exercise_______Date: ___________ LI ______________________ Unique __________ Day: __________ Room: _______ Lab Start Time ________ BIO205L Fall 2011 Exercise 2 Analysis To be completed as a group and turned in at the beginning of your next laboratory period. Include your “Data & Results”, sketches, acquired digital images, etc. as directed by your laboratory instructor. Show your work for all calculations and/or print your MS Excel data sheets. Be sure to include proper units where necessary. Adhere to University’s Honor Code and course policies. Balance between brevity and completeness. 1. Answer the following regarding the Olympus CX31 brightfield microscope you used for this exercise. (a.) Does it have a built-in illuminator or does it have a separate illumination source? (b.) Is it a monocular, binocular, or triocular microscope? (c.) How do adjustments of the field diaphragm affect the quality of a viewed specimen? (d.) How do adjustments of the aperture diaphragm and light intensity affect the view of a specimen? (e.) How does the “field of view” change as magnification is increased? How does this relate to the fact that one should always use the low-power scanning objective first to locate a specimen? (f.) What happens to the “depth of focus” as magnification is increased? (g.) What is used to clean optical surfaces of microscopes and why? 2. List the steps required to adjust the microscope for Köhler illumination. 3. Report in tabular form, for each of the objectives (4 x 10 x, 40 x, x ), the corresponding N.A and total magnification. Calculate the theoretical resolution of the objectives of the student Olympus microscope using Abbe’s equation (show your work, and indicate the wavelength of light chosen). Discuss the relationship of resolving power to numerical aperture. Objective Objective Ocular Total  Resolution  (µm) Magnification NA Magnification Magnification { λ =336 nm} 4X 10X 40X 100X Objective Objective Ocular Total  Resolution  (µm) Magnification NA Magnification Magnification { λ =500 nm} 4X 10X 40X 100X Page 1 of 5
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EXERCISE 1 Page 2 of 5 4. Use the research-grade Olympus microscopes, Scion image software, and their cameras. (a.)
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This note was uploaded on 01/30/2012 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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Ex02_Analysis_Questions - Group Members: _Exercise_Date: _...

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