bio 205l analysis 2 - 1. A. The Olympus CX31 brightfield...

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1. A. The Olympus CX31 brightfield microscope used in this lab does have a built-in illuminator. b. It is a binocular microscope. c. Adjustments to the field diaphragm control the amount of light from the illuminator that will hit the specimen, so it affects the illumination of the specimen. d. If the aperture diaphragm were made smaller, then the light coming in from the illuminator will scatter because only certain angles will be able to hit the specimen plane and go through the objective lens; this allows better contrast. A greater scattering contrast increases the different intensities that our eyes detect on the specimen. This lowers resolution because closing the aperture diaphragm lowers the numerical aperture, which then increases the distance needed to be able to view two objects as separate entities. e. The field of view becomes smaller as magnification is increased. This means you should use the low power objective because the field is larger and you can see the specimen better. f. The “depth of focus” decreases as magnification increases because of aberration that causes “fuzziness” and makes it harder to view two objects close together as separate entities. g. Use provided lens paper because any other material could have elements in it that would scratch the lens. 2. Start with your specimen in focus under the 10x objective lens. First, close the field diaphragm so that only a part of the field is illuminated. Next, turn the condenser height adjustment knob so that the leaflets of the field diaphragm are in focus; do not use the microscope focus knobs. Then, turn the condenser centering screws until the leaflets of the field diaphragm are in the center of the viewing field. Finally, expand the field diaphragm until the entire field is illuminated 3. Objective Objective Ocular Total  Resolution  (µm) Magnification NA Magnification Magnification { λ =336 nm} 4X 0.10 10X 40X 2.056 10X 0.25 10X 100X 0.8225 40X 0.65 10X 400X 0.3164 100X 1.25 10X 1,000X 0.1641 Objective Objective Ocular Total  Resolution  (µm) Magnification NA Magnification Magnification { λ =500 nm} 4X      0.10 10X 40X 3.060 10X 0.25 10X 100X 1.224 40X 0.65 10X 400X 0.4708 100X 1.25 10X 1,000X 0.2448
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0.612*336nm/0.10=2.056nm 0.612*500nm/0.10=3.060nm
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bio 205l analysis 2 - 1. A. The Olympus CX31 brightfield...

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