Ex04LabPractPSKEY - Bio 205L EXERCISE 4 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory If the

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Bio 205L EXERCISE 4 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). 1. Section 4.1: Measurement of Growth Kinetics of Vibrio natriegens a. Properly label the flask of nutrient broth medium before placing it in the incubator-shaker. b. Learn how to operate the “set”, “start”, and “time elapsed” controls of the timer. c. Learn how to operate the spectrophotometer, including turning it on, warming it up, setting wavelengths, taking readings, and auto-zeroing it. d. Wipe the sides and bottoms of cuvettes with a kimwipe prior to reading optical density. e. Know how to correctly position cuvettes in the spectrophotometer. f. Understand what “optical density” is measuring. 2. Section 4.2 : Light Microscopic Observations of Wet Mounts of V. natriegens and E. coli a. Review proper procedure for handling, transporting, and operating light microscopes. b.
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This note was uploaded on 01/30/2012 for the course BIO 205L taught by Professor Hanson during the Spring '07 term at University of Texas at Austin.

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Ex04LabPractPSKEY - Bio 205L EXERCISE 4 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory If the

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