Ex05LabPractPSKEY - KEY: Bio 205L EXERCISE 5 Prep Sheet for...

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KEY: Bio 205L EXERCISE 5 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). 1. Section 5.1 : Observations of Sea Urchin Eggs and Sperm a. Prepare a semi-permanent wet mount of sea urchin sperm in a drop of sea water. b. Prepare a wet mount of sea urchin eggs. c. Observe, sketch, and label cells at oil immersion high magnification. d. Determine the size of each type of cell in reticule units and actual length in nm. 2. Section 5.2: Observations of Sea Urchin Fertilization and Embryo Development a. Prepar e a 1:100 dilution of sea urchin sperm in a final volume of 1000 µL and label it properly. b. Prepare two semi-permanent wet mounts of fertilized eggs. c. Make observations of fertilization and of post-fertilization events every 30 minutes. d.
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This note was uploaded on 01/30/2012 for the course BIO 205L taught by Professor Hanson during the Spring '07 term at University of Texas at Austin.

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Ex05LabPractPSKEY - KEY: Bio 205L EXERCISE 5 Prep Sheet for...

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