Ex06LabPractPSKEY

Ex06LabPractPSKEY - KEY: Bio 205L EXERCISE 6 Prep Sheet for...

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KEY: Bio 205L EXERCISE 6 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). Section 6.1: Isolation of Chloroplasts a. Prepare a Miracloth filter. b. Weigh and blend torn leaf blade tissue. c. Filter the slurry through cheesecloth and the Miracloth filter. d. Follow proper cleaning procedures for the beaker, funnel, and blender to immediately remove green residue so that cell residue does not permanently stick to the glass. e. Balance the tubes with the chloroplast supernatant on the two-pan balance. f. Centrifuge at RCF of 1100 x g to pellet Class II chloroplasts. g. Follow proper cleaning procedures for the centrifuge tubes to remove green residue. h. Use a tissue grinder and Teflon® pestle to homogenize the green liquid suspension. i. Prepare a wet mount slide of the chloroplast preparation. Section 6.2:
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This note was uploaded on 01/30/2012 for the course BIO 205L taught by Professor Hanson during the Spring '07 term at University of Texas.

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Ex06LabPractPSKEY - KEY: Bio 205L EXERCISE 6 Prep Sheet for...

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