Ex07LabPractPSKEY - KEY: Bio 205L EXERCISE 7 Prep Sheet for...

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KEY: Bio 205L EXERCISE 7 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). Section 7.1: Preparation of Crude Protein Extracts from Bovine Liver and Tripe Tissue. a. Using liquid N 2 to keep tissue frozen while grinding it to a fine powder in a mortar and pestle. b. Quickly transferring dry powdered tissue to an ice cold Dounce Homogenizer using a chilled spatula. c. Proper procedure for grinding powdered tissue and keeping it cold in a Dounce homogenizer. Section 7.4: Preparation of Standard Protein Solutions by the Parallel Dilution Method a. Proper use and operation of the spectrophotometer. b. Preparation of a series of protein solutions of known concentration from a stock solution by making parallel dilutions. c. Proper use and application of the formula C1 X V1 = C2 X V2 Section 7.5: Quantification of Total Protein Using the Bradford Assay a. Proper use and operation of the spectrophotometer. b.
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This note was uploaded on 01/30/2012 for the course BIO 205L taught by Professor Hanson during the Spring '07 term at University of Texas at Austin.

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Ex07LabPractPSKEY - KEY: Bio 205L EXERCISE 7 Prep Sheet for...

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