Ex09LabPractPSKEY - KEY: Bio 205L EXERCISE 9 Prep Sheet for...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
KEY: Bio 205L EXERCISE 9 Prep Sheet for LABORATORY PRACTICAL EXAM Procedures and Techniques learned during this laboratory: If the same technique is used in multiple sections of a lab, it does not need to be listed multiple times. ( For the lab practical exam, be sure you know how to properly perform each technique). Section 9.1: Primary Antibody Binding to the Blocked Membrane b. Know how to wash the nitrocellulose membrane and that it is done to remove unbound primary antibodies. c. Each wash cycle consists of steps 1 through 3 below: 1. Add 10 mL of Tris-buffered Saline (TBS) wash solution to the dish that contains the nitrocellulose filter. 2. Return the membrane to the rotary shaker for 5 minutes. 3. Discard the TBS wash solution. 4. Repeat steps 1-3 five times. Section 9.2: Secondary Antibody Binding to the Primary Antibody. a. Add the Secondary Antibody Solution to the washed nitrocellulose and incubate on the shaker for 1.5 hours.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

Ex09LabPractPSKEY - KEY: Bio 205L EXERCISE 9 Prep Sheet for...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online