MCB120L Expt 5 postlab qs

MCB120L Expt 5 postlab qs - Kevin Ma MCB 120L: Lindsay May...

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Kevin Ma MCB 120L: Lindsay May 12, 2010 Post Lab 5 A. SDS-Page 1. MW (g/mol) log MW Migration Distance (cm) Rm (mm band/ mm dye front) Bovine serum albumin 66,000 4.820 0.8 0.131 Ovalbumin 45,000 4.653 1.3 0.213 Carbonic anhydrase 29,000 4.462 2.0 0.328 B-lactoglobulin 18,400 4.265 2.8 0.459 Lysozyme 14,300 4.155 3.15 0.516 Bovine heart LDH 36,000 4.556 1.7 0.279 Porcine heart LDH 36,000 4.556 1.7 0.279 Purified LDH 33,800 4.529 1.8 0.295 Dye front XXX XXX 6.1 XXX 2. Non-Stained Protein Standard Rm vs log MW y = -0.5923x + 2.9774 R 2 = 0.9976 0 0.1 0.2 0.3 0.4 0.5 0.6 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 log MW Rm Series1 Linear (Series1) 3. Sample calculation for bovine heart LDH and porcine heart LDH
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y = -0.5923x + 2.9774 0.279 = -0.5923x + 2.9774 X = logMW = 4.558 MW = 36,000 g/mol Purified LDH MW = 33,800 g/mol 4. (36,000 daltons + 36,000 daltons + 33,800 daltons) / 3 = 35,300 daltons (140,000 daltons) / (35,300 daltons) = 3.97 subunits 4 subunits 5. Our purified LDH sample was somewhat pure, because by far the most significant
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MCB120L Expt 5 postlab qs - Kevin Ma MCB 120L: Lindsay May...

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