BMZ Lab Report C-Fern

BMZ Lab Report C-Fern - 1 Farley Fernsmart Module BMZ Lab...

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1 Farley Fernsmart Module BMZ Lab Section BA Lenna Odstrodka 28 November 2011 My Ferntastic Lab Report
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2 Hypothesis: The hypothesis tested was that the “M” strain of spores could not send a signal for growth when in the petri dish. Methods: This lab began with a basic review of the life cycle of a fern, and an exploration of the life stages involved. Sketches and noes were then made of the fern life cycle. Next, groups of four or five were made, and the instructor passed out petri plates containing both “M” and “W” strains grown at medium density for observation. Observations and data were recorded, and from this the hypothesis given above was developed. Next, the instructor provided a complete set of “M” and “W” strains, which were grown at three different population densities of Low, Medium, and High Density, with approximately nine, 38, and 150 gametophytes per plate respectfully. From these three plates, a data set was collected by removing the lid of each plate, and placing them underneath the dissecting microscope individually. From there, the different shapes of each spore were taken into account, and the number of each shape in each dish was recorded. The shapes were found to be “mitten-shaped” and “light bulb-shaped.” From there, a graph was constructed in the lab workbook in Table 6-2, page 77. The dependent variable was the average percentage of light bulbs, while the independent variable was density. The graph was then examined, and the class discussed causation for the results. The experiment was then set up, with varying filtrates being combined with varying spores in petri plates to test the hypothesis. “M” filtrate was combined with “W” spores as the experimental control, water was put with “W” spores as a manipulated variable, “W” filtrate was put with “W” spores as a positive control, and finally “M” filtrate was
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3 Farley put with “M” spores as a negative control. Then, Table 6-4 in the workbook was filled out with the information given above. After a week had passed, the second part of the procedure began with the petri plates being retrieved from the instructor and the data collected regarding the growth of spores and the shapes they took. These results were then graphed, and the initial hypothesis that the “M” strain of spores could not send a signal for growth when in the petri dish, was rejected. A summary of the data was then
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BMZ Lab Report C-Fern - 1 Farley Fernsmart Module BMZ Lab...

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