lab session 5 - • 4 tubes 9 ml sterile water for serial...

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Cultivation of Bacteria EX:11 EX:12
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Isolation of Bacteria Pure culture: Contain one kind of microbe. We isolate bacteria to study: Growth characteristics Pathogenicity Metabolism Antibiotic sensitivity pattern
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Isolation of Bacteria Three techniques: a) Streak plate b) Spread plate c) Pour plate
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Isolation Methods Streak plate technique: Most common.
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Isolation Methods Spread plate technique: Pour plate technique: Both need serial dilution. Both are quantitative: cfu/ml of the sample. cfu/ml = # of colonies × dilution (2.96×105)
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Serial Dilution
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Spread Plate Procedure
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Pour Plate Procedure
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Pour plate & Spread plate
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Materials Provided Mixed culture. Two nutrient agar plates for streaking method. Three petri-plates and melted agar for
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Unformatted text preview: • 4 tubes 9 ml sterile water for serial dilution. • Pipette bulbs. Special Media for Isolation • Types of microbiological media: • Enrichment media: enhance growth of wanted bacteria. • Selective media: inhibit growth of unwanted. • Differential media: allow you to distinguish one bacterium from another by how they change the media. Different Media • EMB Agar Mannitol Salt Nutrient Agar Materials Provided • Media: EMB, Mannitol salt & nutrient agar one plate each. • One divided plate with the three media for the unknown. • Four cultures: E.c., Ps. a., M.l., Staph. epi . • One unknown culture for identification to be inoculated in the divided plate....
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