Unformatted text preview: B.Sc. in Environmental Science
Course Listing Existing Course On-line Section? Course Name: Course Number: Pre-Requisite(s): Course Category: Semester Offered: First Year Taught: Year in 4-year Syllabus: Credit Hours: Laboratory? Department: College: Instructor (Name): Rank: Short Catalog Listing: The course provides an overview of the aspects of history and classification of microorganisms( Bacteria, Fungi, Algae, Viruses). Functional anatomy of Prokaryotes. Microbial growth, metabolism, and genetics. Medical, Environmental, and Industrial Microbiology. Readings from a required text are Detailed reinforceOutline: used to Course and supplement lecture information. A. Major areas to be covered in lectures are:
Yes No Biological and Environmental Sciences Arts and Sciences Dr. Roda Fahad Al-Thani Associate Professor Yes No Microbiology BIOL 241 BIOL 101 BIOL 102 CORE Compulsory Spring 2010 Second 3 1) Introduction and brief history of microbiology. 2) Functional anatomy of prokaryotic (Bacteria) cell. 3) Microbial metabolism. Course Objectives and Learning Outcomes: Students must be able to: 4) Microbial growth. 5) The control of microbial growth. 1)Understand the structure and function of microorganisms. 6) Microbial genetics. 2) Know the classification of Prokaryotic and Eukaryotic microorganisms. 7) Biotechnology and recombinant DNA. 3) Recognize the basis for microbial growth, metabolism, and genetics. 8) Classification of microorganisms. 4) Comprehend principles of medical, environmental, and industrial 9) The Prokaryotes: Domains Bacteria and Archaea. microbiology. 10) The Eukaryotes: Fungi, Algae, Protozoa,.. B.Sc. in Environmental Science
Assessment of Student Progress and Performance: 1. A pre-test and post-test instrument to determine the extent of student improvement during the semester. 2. In the following categories: First Exam. 15% Second Test Questions: Example Exam. 15% Final Exam. 35% Dr. Roda F. AL-Thani Name: Qatar University Microbiology Research Paper and Presentation 5% Biological sciences Dept. 1062241 Lab 30% 9-4-2006 First Exam
(15 points) B 1- Put () in front of the True sentence and (x) in the False A. Reading: sentence. ( 1) ) 1- The Cyanobacteria survive without the cell membrane. ( ) 2- Peptidoglycan is a component of the bacterial cytoplasm. 2) ( ) 3- Bacteria lack mitochondria in the cell cytoplasm. ( 3) ) 4- The generation time is the same for all bacterial species. ( ) 5- Prokaryotes and eukaryotes differ primarily with regard to the absence or presence of a cell wall. ( ) 6-Carbon dioxide is fixed into organic compounds by photoautotrophs and B. Writing: chemoautotrophs , these organic compounds provide nutrients for chemoheterotrophs. ( ) 7-Almost all of extreme thermophiles are archaebacteria. 1) ( ) 8- Koch's use of a pure culture ensured that only one type of bacterium was involved in the cause of disease. 2) ( ) 9- All microorganisms are pathogenic. ( ) 10- Enzymes are biological catalysts. ( ) 11- If a substance is oxidized, it gains electrons. C. Listening: ( ) 12- The two major components of the bacterial cell membrane are lipid and carbohydrate. 1) ( ) 13- Gram- positive bacteria are those that retain the crystal violet-iodine complex when a decolorizing agent is added, appearing blue-purple at the conclusion of the 2) procedure. ( ) 14- Protista are prokaryotes. ( ) 15- In a complex growth medium, the identity and quantity of each component is D. Critical Thinking: known, but in a synthetic medium they are unknown. ( 1) ) 16- Metabolism is common to all eukaryotic organisms only. ( )17-In oxidation-reduction reactions, energy is derived from the transfer of electrons. ( )18-In a nomenclature systems designed by Carolus Linnaeus (1735), each living organism is assigned two names. ( E. Computer Literacy: use inorganic compounds as their energy sources and carbon )19-Chemoautotrophs dioxide as their carbon source. ( 1) )20-Louis Pasteur demonstrated that microorganisms are in the air everywhere and offered proof of biogenesis (1861). B.Sc. in Environmental Science
2. Multiple Choice
1-Koch's postulates provided guidelines for a) relating certain microorganisms with certain diseases. b) sterilizing laboratory supplies. c) preparing pure cultures. d) testing the sensitivity of microbes to antibiotics. 2-The bacillus, coccus, and spirillum shapes are typical of the a) fungi. b) bacteria. c) viruses. d) protista. 3-Penicillin was discovered by a) Waksman. b) Domagk. c) Fleming. d) Ehrlich. 4-In the log phase of growth a) the death rate is equivalent to the reproductive rate. b) spore formation is a common event. c) the number of cells in the population increases rapidly. d) the growth curve is at a plateau. 5-An acidophile would grow best at a pH of a) 2. b) 6. c) 9. d) 12. 6-An organism having pili has the advantage over one that lacks pili because a) it can move actively. b) it can obtain nutrients from beef broth. c) it can be stained easily. d) it can attach to specific surfaces. 7-Facultative bacteria are those that a) grow on natural and synthetic media. b) produce spores or capsules, but not both. c) grow in the presence or absence of oxygen. d) posses cell membranes but no cell walls. 8-Monotrichous and peritrichous bacteria differ in a) their nutritional requirements. b) layers of the cell wall. c) spores formed per cell. d) number and position of flagella. 9-A selective medium is one that a) allows one type of bacterium to grow while inhibiting another type. b) permits bacterial rods to grow while inhibiting cocci. c) contains nutrients on which only bacteria can grow. d) distinguishes colonies of one type of bacterium from those of another type. 10-Which one of the following is not a variation of cocci arrangement? a) Spirococcus b) Sarcina B.Sc. in Environmental Science
c) Diplococcus d) Streptococcus 11-A mesophilic bacterium could a) reproduce at 37 C b) produce human disease. c) grow at human body temperature. d) All the above (a-c) are correct. 12-The process by which bacteria reproduce is known as a) meiosis. b) splitting. c) mitosis. d) binary fission. 13-Lipopolysaccharide (LPS) is found in the a) gram-negative outer membrane. b) gram-positive cell wall. c) periplasmic space. d) inclusion granules. 14-Small molecules of DNA that exist in bacteria as independent circular units are known as a) chromosomes. b) nucleoids. c) plasmids. d) nucleoli. 15-Spherical organisms arranged in irregular groups of cells that resemble bunches of grapes are referred to as a) staphylococci. b) streptococci. c) sarcinae. d) cocci. 16-Enzymes play an important role in biological chemistry because they a) break down to release metal ions. b) lower the amount of activation energy required for a reaction. c) raise the ionic strength of a water solution. d) retain the pH at the neutral level preferred by most microorganisms. 17-ATP releases its energy by a) activating its protons to form ions. b) absorbing heat from the environment. c) combining with other ATP molecules. d) splitting into a phosphate and ADP. 18-Fermentation differs from aerobic respiration because a) fermentation does not require that glucose be present. b) fermentation does not result in a net ATP gain. c) fermentation does not result in pyruvic acid production. d) fermentation does not require that oxygen be present. 19- Inorganic ions that compose parts of enzymes are referred to as a) cytochromes. b) substrates. c) cofactors. d) intermediates. 20-Which of the following is a distinguishing characteristic of prokaryotic cells? a) True nucleus B.Sc. in Environmental Science
b) They have cell walls containing peptidoglycan c) Cell division by mitosis d) Ribosomes are larger size and smaller size 21-You have isolated a motile, gram-positive cell with no visible nucleus. You can assume this cell has a) Ribosomes b) Mitochondria c) A Golgi complex d) All of the above 22-In aerobic respiration: a) O2 functions as the final electron acceptor. b) Organic compound functions as the final electron acceptor. c) Inorganic compound functions as the final electron acceptor. 3. Fill in the following blanks
1-In______________Life forms arise from nonliving matter. 2-______________Population of a single species without other species present. 3- _____________Procedure to cultivate microorganisms as individual species. 4-The indirect methods of microbial growth are 1_________________,2_________________,and 3_____________________ 5-_______________are sometimes called fimbriae. 6-________________ refers to chemical reaction that results in the breakdown of complex molecules and usually release energy. 7- _________________are oxygenic phototrophs bacteria. 8-_________________is the most common pathway for the oxidation of glucose and pyruvic acid is the end product. 5-Lable the following numbers. B.Sc. in Environmental Science With Best of Luck Qatar University Biological sciences Dept. General Microbiology
( 2h. 35 points) 2-1-2006 Dr. Roda F. AL-Thani Name: Final Exam
322241 1. Multiple Choices
1-Which of the following is the dominant type of immunoglobular protein A-IgG B-IgM C-IgA D-IgE 2-Prokaryotic organisms are classified into two domains A-Archaea and Eukarya B-Bacteria and Eukarya C-Eukarya and Prokarya D-Archaea and Bacteria 3-Which of the following is not true about scientific nomenclature? A-Each name is specific and consist of a genus and specific epithet B-The names are standardized C-It was first designed by Linnaeus D-Names vary with geographical location 4-Bacterial species are differentiated by A-Morphology and source of energy B-Chemical composition and biochemical activities C-Nutritional requirements D-All of the above B.Sc. in Environmental Science
5-A group of bacteria derived from a single cell is called strain and closely related strains constitute a bacterial A-Genera B-Families C-Orders D-Species 6- are infectious pieces of RNA that cause some plant diseases A-Prions B-Oncogenic viruses C-Viroids D-virion 7-A base substititution occurs when A-One base pair in DNA is replaced with a different base pair B-One or a few base pairs are deleted from DNA C-More than one base pairs in DNA is replaced with a different base pairs D-One or a few base pairs are added to DNA 8- is the study of the transmission, incidence, and frequency of disease A-Pathology B-Immunology C-Epidemiology D-Phycology 9-Most bacteria are in diameter and in length A-0.2-2.0m , 2-8m B-0.9-9.0m , 20-80m C-0.02-0.2m , 0.2-2.0m D-Non of the above 10-The percentage of base pairs in the nucleic acid of cells can be used in the classification of organisms A-G + C B-A + T C-T + C D-A + G 11-may be classified on the basis of morphology as crustose, foliose , or fruticose A-Bacteria B-Fungi C-Lichens D-Algae 12-Information contained in the DNA is A-Transcribed into RNA and translated into proteins B-Transcribed into DNA and translated into RNA C-Transcribed into DNA and translated into proteins D-Translated into proteins and transcribed into DNA 13-The main material of fungal cell walls is A-Chitin B-Glucan and mannan C-Glycocalyx D-Peptidoglycan 14-Most bacteria grow best at a pH value between A-6.5 and 7.5 B-2.5 and 3.5 B.Sc. in Environmental Science
C-5.5 and 10 D-1 and 7 15-Extreme halophiles, extreme thermophiles, and methanogens are included in the A-Archaea B-Bacteria C-Eukarya D-All of the above 16-are unicellular and have pectin and silica cell walls; some produce neurotoxin A-Green algae B-Red algae C-Brown algae D-Diatoms 17- is the genetic composition of an organisms , its entire complement of DNA A-Phenotype B-Genomics C-Genetic engineering D-Genotype 18-Members of the phylum are gram-negative and nitrogen-fixing bacteria A- Proteobacteria B- Proteobacteria C- Proteobacteria D- Proteobacteria 19-Prokaryotic relation ships are determined by A-rRNA sequencing B-DNA sequencing C-mRNA sequencing D-tRNA sequencing 20-In the presence of certain chemicals , cells synthesis more enzymes. This process is called A-Induction B-Repression C-Mutation D-Translation 21- mycoses are fungal infections deep within the body that affect many tissues and organs A-Systemic B-Subcutaneous C-Cutaneous D-Superficial 22-The utilization of nitrogen gas as a source of nitrogen is called A- Nitrogen fixation B- Nitrogen reduction C-Nitrogen oxidation D-Nitrogen assimilation 23-The first step of dissimilative nitrate reduction involves A-Nitrate reductase B-Nitrite reductase C-Nitrate oxidase D-Nitrite oxidase 24-In waste water treatment, primary treatment consists of B.Sc. in Environmental Science
A-Physical separations only B-Chemical separations only C-Biological separations only D-All of the above 25-In nonspecific resistance, which of these is the second line of defense A-Phagocytic white blood cells B-Antibodies C-Intact skin D-Normal micro biota 26-Which of the following is a distinguishing characteristic of prokaryotic cells? A-True nucleus B-They have cell walls containing peptidoglycan C-Cell division by mitosis D-Ribosomes are larger size and smaller size 27-You have isolated a motile, gram-positive cell with no visible nucleus. You can assume this cell has A-Ribosomes B-Mitochondria C-A Golgi complex D-All of the above 28-Species of Bacillus and Streptomyces have significance in industry as A-Producers of steroid products. B-Starter cultures in the fermentation of distilled spirits. C-Producers of antibiotics. D-Starter cultures for yogurt production. 29-Escherichia coli is a good indicator of water pollution because this bacterium A-Grows only in high-salt environments. B-Is commonly found in the human intestine. C-Is resistant to most antibiotics. D-Undergoes recombinations readily. 30-The primary decomposers of dead organic matter are microorganisms such as A-Protozoal cysts and hepatitis A viruses. B-Multicellular parasites and rickettsiae. C-Chlamydiae and viroids. D-Bacteria and fungi. 31-The biochemical oxygen demand (BOD) is a measure of the A-Amount of biological pollution in water. B-Degree of chemical pollution in water. C-Number of hepatitis viruses in underground springs. D-Effectiveness of fluoridation in the preparation of drinking water. 32-The high protein content of legume plants may be related to A-The presence of sulfur-reducing bacteria in the stems of the plants. B-The rapid conversion of carbohydrates to proteins after picking. C-The activity of nitrogen-fixing bacteria in the root nodules. D-Photosynthesis taking place in the leaves. 33-The cyclic transformation of sulfur is important because sulfur is an essential component of A-Steroids such as cholesterol. B-Nucleic acids such as DNA. C-Enzymes such as phosphatase. B.Sc. in Environmental Science
D-Amino acids such as cystine. 34-Which one of the following is the correct sequence used in the purification of water? A-Sedimentation chlorination filtration. B-Flocculation filtration chlorination. C-Sedimentation flocculation chlorination. D-Chlorination flocculation filtration. 35-Legumes develop symbiotic relationships with nitrogen-fixing bacteria belonging to the genus A-Nitrosomonas. B-Nitrobacter. C-Rhizobium. D-Azotobacter. 36-Which one of the following is not a free-living species involved in nitrogen fixation? A-Bacillus B-Rhizobium C-Azotobacter D-Pseudomonas 2. True or False
( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ) 1- Transient microbiota are microbes that are present for various periods and then disappear. ) 2- Phylogeny is the evolutionary history of a group of organisms. ) 3- During the log phase, the bacteria multiply at the faster rate possible under the conditions provided. ) 4- Lichens are used for their pigments and as air quality indicators. ) 5- Fatty acid profiles can be used to identify some organisms. ) 6- Transposons are small segments of DNA that can move from one region to another region of the same chromosome. ) 7- Pleomorphic bacteria can assume several shapes. ) 8- Purple and green photosynthetic bacteria are photoautotrophs that use light energy and CO2 and do not produce O2. ) 9- The cell walls of many algae and some fungi contain cellulose. ) 10- Basidiomycota have septate hyphae and produce basidiospores. ) 11- A nosocomial infection is any infection that is acquired during stay in any health facility. ) 12- Mesosomes, irregular infoldings of the plasma membrane, are artifacts, not true cell structures. ) 13- Any bacterial genes can be transferred by transduction. ) 14- An indirect way of estimating bacterial numbers is measuring the metabolic activity of the population. ) 15- Living organisms are currently classified into five domains. ) 16- All phyla of gram-negative bacteria are related phylogenetically to the proteobacteria ) 17- Cyanobacteria are photoautotrophs that use light energy and O2 and do produce CO2. ) 18- Algae are classified according to their structures only. ) 19- The protein coat surrounding the nucleic acid of a virus is called the envelope. ) 20- Opportunistic pathogens cause disease under normal conditions. ) 21- Staphylococus aureus and Clostridium are producing endotoxins ) 22- The ability to ward off disease through body defenses is called resistant B.Sc. in Environmental Science
( ) 23- Hydrogen sulfide (H2S) is used by autotrophic bacteria, the sulfur is oxidized to form S or SO2-4 . ( ) 24- Sludge digestors work because microorganisms are present. ( ) 25- The polysaccharide capsule of Streptococcus pneumoniae prevents phagocytosis by the host's immune system. ( ) 26- Inflammation is characterized by redness, pain, heat, swelling and some time the loss of function. ( ) 27- The cyclic transformation of nitrogen is important because nitrogen is an essential component of nucleic acids and amino acids. ( ) 28- Symbiotic fungi called mycorrhizae live in and on plant roots. 3. Fill in the following blanks
1-_________________ is the scientific study of disease and the effects of disease. 2-_________________ is the scientific study of fungi. 4-The use of microorganisms to remove pollutants is called _________________. 5-Elements are _______________ and ______________ by microorganisms during biogeochemical cycles. 6-Microorganisms play role in food production for example in_____________,________, _____________and____________) and in industrial products for example (___________ and _____________). 7-The three basic bacterial shapes are _____________,______________ and _______________. 8-______________are self-replicating circular molecules of DNA carrying genes those are not usually essential for the cell's survival 9-_____________and__________________are types of vaccines 10-____________________is the science of the classification of organisms also provides a means of identification organisms. 11-__________________are antiviral proteins produced in response to viral infection. 12-__________________is the movement of water from areas of high to low concentration across a selectively semipermeable membrane until equilibrium is reached. 13-__________________is the time required for a cell to divide or population to double. 14-__________________is a chemical substance that cause the body to produce specific antibodies. 15-Direct measurement of microbial growth are: 123416-The most important phyla of fungi are: B.Sc. in Environmental Science
1324- 17-The portals of entry for microorganisms to the body are: 123418-The first lines of defense are: 123- 4-Matching
A-Match each term with its correct characteristic.
( ( ( ( ( ( )Bacteria )Archaea )Fungi )Algae )Viruses )Mycoplasma (1)is a bacteria genus that naturally lacks cell walls. (2)are not placed in a kingdom. (3)are eukaryotic; most are phoyoautotrophs. (4)are aerobic or faculatively araerobic chemoheterotrophs. (5)have pseudomourein, they lack peptidoglycan. (6)unicellular, and most of them multiply by binary fission. B-In bacterial cell, match the structures to their functions
____ Cell wall ____ Endospore ____ Fimbriae ____ Flagella ____ Glycocalyx ____ Pili ____ Plasma membrane ____ Ribosomes abcdefghiattachment to surfaces cell wall formation motility protection form osmotic lysis protection from phagocytes resting protein synthesis selective permeability transfer genetic material C-Nitrogen Cycle Process
( , )Ammonification ( , )Nitrification ( , )Denitrification ( , )Nitrogen fixation Chemical reaction
(1)N2-------------Ammonia (NH3) (2)NO3-----------N2 (3)NH4----------- NO2 NO2-------------NO3 (4)Amino acids ------- Ammonia (NH3) Microorganisms
(a)Aerobic and anaerobic bacteria (b)Nitrosomonas Nitrobacter (c)Pseudomonas (d)Rhizobium D-Match the disease with the causative agent and the infected system.
( , )Gonorrhea ( , )Aflatoxin poisoning ( , )Tuberculosis (1)Maycobacterium tuberculosis (2)Bacillus anthracis. (3)Nisseria gonorrhoeae (a)Nervous (b)Respiratory (c)Digestive B.Sc. in Environmental Science
( , )Leprosy ( , )Anthrax (4)Aspergillus flavus (5)Mycobacterium leprae (d)Lymphatic (e)Reproductive Quiz 1
1-Label the following numbers. 2- Fill in the following blanks
1-________________ refers to chemical reaction that results in the breakdown of complex molecules and usually release energy. 2-_________________are oxygenic phototrophs bacteria. 3-_________________is the most common pathway for the oxidation of glucose and pyruvic acid is the end product. 3-Multiple Choice
1- In fermentation process: A-Energy releases from sugars or other organic molecules by oxidation. B- Energy releases from sugars or other organic molecules by reduction. C- Energy releases from inorganic molecules by oxidation. 2- In anaerobic respiration: A-O2 functions as the final electron acceptor. B-Organic compound functions as the final electron acceptor. C-Inorganic compound functions as the final electron acceptor. 3- The end products for Saccharomyces in pyruvic acid fermentation are: A-Propionic acid, acetic acid, CO2 and H2. B-Propionic acid and lactic acid. C-Ethanol and CO2. B.Sc. in Environmental Science
Examples of Assessment of Student Performance: 1. In relation to program objectives 2. In relation to the following specific categories: A. Reading example 1 example 2 etc. Scoring Guide and Criteria: Excellent (90 to 100%) . example 1 - well written, grammatically correct, flawless report B. Writing . example 2 - master's operation of laboratory analytical equipment Examples of Feedback to Students: For example: past exams, sample questions, summary of grades etc C. Listening Very GoodThinking D. Critical (80 to 89%) Resources: Textbooks:Microbiology an introduction 9th.ed. Tortora, Funke, and Case, Good (70 to 79%) 2007, Benjamin Cummings. Brock biology of microorganisms . Madigan, Martinko, and Parker 9th ed. 2000. Borderline (60 to 69%) Webpages: www.microbiologyplace.com Access for Special Needs Students: Fail (<59%) Students should bring any special needs to the attention of the instructor at the first day of the text book Other: CD withclass. Faculty are aware of the process for dealing with these needs (see email circulated November 2007 below). Broadcast Email Student Guide for Successful Completion of this Course: 1) Come to Needs Office From: Special class every time it meets; and become recognized by the instructor (for all of the correct reasons). To: All Faculty 2) Come to class prepared to listen, write, engage, ask questions, and learn sitting in a lecture hall and taking Special part of Subject: Exams for Students with notes is Needs the learning process.
3) Do not come late for class it is disruptive and shows a lack of respect; you also miss content. Please note that students with special needs who are provided with readers 4) Past a quiet room during their exams. Wewho pleased to the night before the require experience has shown that students are study only inform you that a quiz/exam, Room for Students with Special Needs may be used as an exam Resources no matter how clever, almost never score above a C on exams. room for students with special needs. We encourage instructors to send exam 5) Reading the textbook usually improves your of timeon exams by a letter questions to us (via: email, mail, or fax) ahead score so that we may arrange grade. necessary preparations. for the 6) Ask questions, engage with the class. 7) Study in groups and by yourself. 8) Cell Phone OFF. B.Sc. in Environmental Science
Honor Code: Academic Misconduct: Submitting plagiarized (stolen) work to meet academic requirements including the representation of another s work or ideas as one s own; the unacknowledged word for word use of another person s ideas; wholesale "cutting and pasting" of material; falsification of documents; inducing others to participate in misconduct; and/or the falsification, fabrication, or dishonest Program Objectives/Outcomes Addressed in this Course: Indicate all that representation in reporting research results are grounds for charges of apply (the objectives and outcomes are provided in the instructions file). academic misconduct. Any form of cheating or other type of academic misconduct this includes attempting to circumvent the scheduled exam b c d e f Objective 1- anda process are grounds for charges of academic misconduct. Any cheating or other b c type of academic a misconduct will be reported to university administration and at Objective 2 minimum will result in automatic failure of the course.
Objective 3 Objective 4 a a b b c c d d e f ...
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