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08_lecture - Chapter 8 Control of Microorganisms in the...

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Chapter 8 Control of  Microorganisms in the  Environment 1
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2 Figure 8.1
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sterilization destruction or removal of all viable  organisms disinfection killing, inhibition, or removal of disease  causing (pathogenic) organisms  disinfectants agents, usually chemical, used for disinfection usually used on inanimate objects 3 Definition of Frequently Used  Terms
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sanitization reduction of microbial population to levels deemed  safe (based on public health standards) antisepsis prevention of infection of living tissue by  microorganisms antiseptics chemical agents that kill or inhibit growth of  microorganisms when applied to tissue 4 More Definitions…
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5 Figure 8.2
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chemotherapy  use of chemicals to kill or inhibit growth of  microorganisms within host tissue agents that kill microorganisms or inhibit their  growth cidal agents kill static agents inhibit growth 6 Antimicrobial Agents
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- cide suffix indicating that agent kills germicide kills pathogens and many nonpathogens  but not necessarily endospores include bactericides, fungicides, algicides,  and viricides 7 -cidal Agents
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- static suffix indicating that agent inhibits growth include bacteriostatic and fungistatic 8 -static Agents
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9 The Pattern of Microbial Death microorganisms are not killed instantly population death usually occurs  exponentially measure of agent’s killing efficiency decimal reduction time – time to kill 90% must be sure persister cells (viable but  nonculturable (VBNC) condition) are dead once they recover they may regain the ability to  reproduce and cause infection 
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10 Figure 8.3 The Pattern of Microbial Death
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population size larger populations take longer to kill than  smaller populations population composition microorganisms differ markedly in their  sensitivity to antimicrobial agents 11 Conditions Influencing the  Effectiveness of Antimicrobial  Agent Activity
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concentration or intensity of an antimicrobial  agent usually higher concentrations or intensities  kill more rapidly relationship is not linear duration of exposure longer exposure   more organisms killed 12 More Conditions…
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