Quiz508 - General Genetics Page 1 of 3 Quiz 5 Form A 1 A...

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General Genetics 3110:211 Fall, 2008 Quiz 5 ID_______________________ Form A 1. A double mutant is generated in the lac operon of E. coli that is both operator constuitive (O - ) and produces a super-repressor (I s ). What will be the phenotypes of this double mutant for lacZ inducibility? ON 2. Which part of the lac operon would be the equivalent of a eukaryotic transcription factor? Repressor or CAP-cAMP 3. How would a deletion of the lacY region of the lac operon affect the control of the lacZ region? It wouldn’t 4. An inversion mutation in E. coli moves the gene for the lac repressor (I) to a location next to the Trp operon. How will this mutation affect the control of the lac operon? No change 5. In the Trp operon if a mutation removed the attenuation sequence from the DNA, which of the following would be possible a. The operon would be always off b. The operon would be always on c. The operon would be off when trp was needed d. The operon would be on when trp wasn’t needed e. The operon would be controlled by glucose levels
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