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Homework2answers - CEE266EnvironmentalBiotechnology...

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Unformatted text preview: CEE266EnvironmentalBiotechnology Homework#2 1. [10points]Whichofthefollowingformsofoxygenismosttoxictolivingorganisms? a. molecularoxygen b. hydroxylradical c. superoxideanion d. hydrogenperoxide 2. [10points]Thedilutionrateofachemostatdependson(multiplecorrectanswers): a. hydraulicresidencetime b. temperature c. incomingsubstrateconcentration d. bacterialyield 3. [10points]Growthisdefinedasanincreasein a. thesizeofindividualorganisms. b. thelifespanofindividualcells. c. thenumberofcoloniespresentinaculture. d. thenumberofcells. 4. [10points]Amicrobegrowinginafunctioningrefrigeratorisprobablya a. psychrophile. b. mesophile. c. thermophile. d. hyperthermophile. 5. [30points]Refertotheattachedpaper:Kampfer,P.&Kroppenstedt,R.M.(2004). Pseudonocardiabenzenivoranssp.nov.,InternationalJournalofSystematicandEvolutionary Microbiology54,749751.Listtencharacteristicsofthisorganism(atleastoneofeachtypeof classification,e.g.,morphological,physiological,chemotaxonomic,andphylogenetic). Answersmayinclude: i. Morphological:Grampositive;rodshaped;whiteaerialmycelium,palevegetative mycelium, ii. Physiological:aerobic;oxidasepositive;growthat2530oC;hydrolysisofphenyl phosphonate;assimilationoftrehalose,ribose,4hydroxybenzoate,serine,mannose, phenylalanine,etc. iii. Chemotaxonomic:majormenaquinoneisMK8(H4),;predominantpolarlipidsare diphosphatidylglycerol,phosphatidylethanolamine,andphosphatidylinositol; phosphatidylcholineabsent;majorfattyacidsareisobranchedhexadecanoateand hexadecanoate. iv. Phylogenetic:16SrDNAsequenceavailableataccession#AJ556156;DNA:DNA hybridization38%similartoP.sulfidoxydans,and23%similartoP.hydrocarbonoxydans. 6. [30points]ConsiderthefollowingdataonthegrowthofPseudonocardiadioxanivorans,P. benzenivorans,andP.antarctica.Ranktheseintheorderofgrowthrateandgrowthyield. Calculategandkforeach.(Hint:plotthesegrowthcurves). Time(h) 0 4 8 12 16 20 24 28 32 36 40 44 48 P.dioxanivorans 0.022 0.043 0.146 0.465 0.843 1.065 1.118 1.124 1.138 1.148 1.142 1.127 1.146 P.benzenivorans 0.031 0.028 0.045 0.058 0.079 0.203 0.418 0.586 0.754 0.876 0.891 0.905 0.923 P.antarctica 0.029 0.033 0.086 0.193 0.344 0.462 0.518 0.559 0.571 0.587 0.593 0.618 0.606 Growthrateandyield:P.dioxanivorans>P.benzenivorans>P.antarctica HOMEWORK # 2 QUESTION # 6 Time 0 4 8 12 16 20 24 28 32 36 40 44 48 n n n avg n g k P. diox 0.022 0.043 0.146 0.465 0.843 1.065 1.118 1.124 1.138 1.148 1.142 1.127 1.146 1.660 0.853 0.335 0.949 4.21 0.07 P. benzi 0.031 0.028 0.045 0.058 0.079 0.203 0.418 0.586 0.754 0.876 0.891 0.905 0.923 1.035 0.484 0.361 0.627 6.38 0.05 P. ant 0.029 0.033 0.086 0.193 0.344 0.462 0.518 0.559 0.571 0.587 0.593 0.618 0.606 0.828 0.423 0.164 0.472 8.48 0.04 Exponential phase hr hr-1 PseudonocardiaGrowthCurves 1.4 1.2 Absorbance 1 0.8 0.6 0.4 0.2 0 0 5 10 15 20 25 Time(h) P.dioxanivorans P.benzenivorans P.antarc@ca 30 35 40 45 50 ...
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This note was uploaded on 02/02/2012 for the course CEE 266 taught by Professor Shailymahendra during the Fall '11 term at UCLA.

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