Open Yale CoursesBENG 100: Frontiers of Biomedical EngineeringLecture 4 - Genetic Engineering (cont.)<< previous session| next session >>Overview:Professor Saltzman continues his presentation on DNA technology by discussing control of gene expressionusing two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniquesto mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria arediscussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzmanexplains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gelelectrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulinexpression in large animals by cloning into their genome the insulin gene with the
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