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Finalkey - Bio 115 Winter 2011 Final Exam March 18 v1 Name...

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Bio 115, Winter 2011 Name__________________ Final Exam, March 18 v1 _____________________________________________________________________________________________________________________________ _ page 1 page total: Instructions: Write your name at the top of every page. Restrict your answers to the space provided. 1. a. Diagram a typical eukaryotic Pol II gene (DNA) sitting in the genome and point out the following features : start codon, stop codon, transcription initiation site, core promoter, termination region, polyadenylation site, three exons, two introns, upstream regulatory elements, and untranslated regions. (5 points) b. Which feature (s) contain sequences that most often regulate the following processes: (5 points) translation initiation 5' UTR transcription initiation core promoter, URE alternative splicing introns and exons mRNA stability 3' UTR 3 end processing Poly-A site +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". (4 points each) For each "true" answer, give an example or explanation that supports the statement. For each "false" answer, explain why the statement is false. 2. DNA polymerase requires an RNA polymerase for DNA replication. True. RNA polymerase is required to lay down the primer from which DNA polymerase will replicate. This is because DNA polymerase requires a 3' end from which to start. 3. A cell infected by a retrovirus cannot be cured. True. Retroviruses integrate their genome into the host chromosome, and all daughter cells will inherit that sequence. 4. Alternative splicing patterns of genes are best determined by comparing genomic and cDNA sequences. True. cDNA would be missing introns and any exons that are spliced out. If two cDNAs are identified for a gene, one with an exon and one missing the exon, which we would know from comparing to genomic DNA, we can conclude the exon is alternatively spliced. (A picture showing this would also be good. Page total: 22 URE intron1 intron2 exon1 exon2 exon3 polyA site termination region 3’UTR 5’UTR core promoter transcription start site stop codon
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Bio 115, Winter 2011 Name__________________ Final Exam, March 18 v1 _____________________________________________________________________________________________________________________________ _ page 2 page total: (True/False continued...) 5. Repair of a break in a gene by the double strand break pathway requires the homologous allele. True. In DSBR, the DNA from the broken chomosome must invade the homologous chromosome, which is used as a template to repair the break. (A drawing of this situation would also be good) (I will also accept false if there is a clear discussion of NHEJ). 6. Telomerase RNA functions as a ribozyme in telomere synthesis. False. A ribozyme has catalytic activity, but telomerase does not have catalytic activity. Telomerase serves as a template for telomerase RT to add on telomere DNA. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Short answer. Limit your answer to the space provided.
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