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Unformatted text preview: Bio 115, Winter 2011 Name__________________ Midterm #1, January 25 page 1 page total: Instructions: Write your name in ink at the top of every page. Do not write on the back of pages. Restrict your answers to the space provided. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Answer the following questions with "true" or "false". (3 points each) For each "true" answer, give an example or explanation that supports the statement. For each "false" answer, explain why the statement is false. 1. The difference in complexity between "simple" eukaryotes (like C. elegans ) and humans is explained by differences in gene numbers. False. It does not scale with gene numbers. The difference is explained by more complex expression pattern and regulation of those genes. (will also accept a discussion of alternative splicing) 2. In order to carry out a PCR reaction, the entire sequence of the target DNA must be known. False. One only needs to know the ends of the sequence in order design primers. 3. A genomic library would not be a good source for cloning sequences that regulate transcription. False. Genomic libraries contain all the sequences of a genome, which includes the sequences that regulate transcription. (or A cDNA library would not be a good source for cloning sequences that regulate transcription because those sequences are not transcribed into RNA) 4. RNA polymerase II fidelity is explained by its selective binding of specific nucleotides. False. RNA Pol II binds all nucleotides, but it only catalyzes addition of nucleotides correctly base paired with template DNA. 5. A gel-shift assay uses a native gel, not a denaturing gel. True. A gel-shift assay is used to look for interactions between different proteins/nucleic acids, so conditions must maintain those interactions. Denaturing gels would disrupt those interactions. 6. Inhibiting RNA Pol III would not affect protein translation. False. RNA Pol III is responsible for transcribing both tRNAs and 5S rRNA, which are required for the translation machinery. Bio 115, Winter 2011 Name__________________ Midterm #1, January 25 page 2 page total: +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Short answer questions. Limit your answer to the space provided. 7. How does the appearance of gene families allow for evolution of more complex organisms? (3 points) Gene families are collections of genes derived from the same ancestor gene. With more than one copy of the gene in the genome, expression patterns and regulation of the gene can change, even as the original function is maintained. This variation allows for more complexity....
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This document was uploaded on 02/05/2012.
- Winter '09