Mid1_08practice_annotated

Mid1_08practice_annotated - Professor Lindsay Hinck Bio 110...

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Professor Lindsay Hinck Bio 110 Fall, 2008 Midterm I Remember to manage your time wisely. You have 35 problems: 5 True/False and 30 multiple choice. This means approximately 3 minutes/ question so you have to move along. Remember to write and bubble in your student ID and name on the scantron sheet. Also you must bubble in under test form which version (A or B) of the test you are taking
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QUESTIONS 1-5) True/False Questions: Mark A for True or B for False (2 points each, total 10 points) 1) A yeast 2-hybrid screen is a technique used to identify proteins that interact with each other. 2) Tagging molecules with glutathione-S-transferase generates an easy way to isolate the proteins since it confers high affinity binding to glutathione. 3) Hydropathy plots are useful for identifying hydrophobic polypeptide segments that are long enough to span a membrane as an alpha-helix or as a beta- sheet. 4) A symporter would function as an antiporter if its orientation in the membrane were reversed (that is, if the portion of the protein normally exposed to the cytosol faced the outside of the cell instead). 5) The resting membrane potential of a typical animal cell arises predominantly through the action of the Na+-K+ pump. 6) Which of the following statements is true about alpha helices. (3 points) A) A hydrogen bond forms between every third side chain. B) The amino acid side chains alternatively project toward the N-terminus and then to the C-terminus giving the chain polarity. C) The N-terminus of the alpha helix resides within the cell. D) The C-terminus has a partial negative charge. E) Polar side chains are buried inside the cylinder of an alpha helix, allowing the single polypeptide chain to go through the membrane.
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QUESTIONS 7-10) Below are choices (A-D) for how the lipid bilayer would be affected under the following conditions. Choose the correct answer from the 4 choices (A-D) for each of the conditions identified below. (3 points each, total 12 points) 7) The hydrocarbons were shorter than normal, say 10 C atoms long. A) More fluid B) Less fluid C) Same fluidity as normal membrane D) Patchy fluidity with some areas being more fluid and some less 8) All the hydrocarbons were saturated. A) More fluid B) Less fluid C) Same fluidity as normal membrane D) Patchy fluidity with some areas being more fluid and some less 9) All the hydrocarbons were unsaturated. A) More fluid B) Less fluid C) Same fluidity as normal membrane D) Patchy fluidity with some areas being more fluid and some less 10) A bilayer composed of a mixture of two kinds of lipid molecule: one with two long saturated hydrocarbon tails (e.g. sphingolipids) and the other with two unsaturated hydrocarbon tails. A) More fluid
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This note was uploaded on 02/06/2012 for the course BIO 110 taught by Professor Rexach during the Fall '08 term at UCSC.

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Mid1_08practice_annotated - Professor Lindsay Hinck Bio 110...

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