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15_lecture - Chapter 15 Recombinant DNA Technology 1 Some...

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Chapter 15 Recombinant DNA  Technology 1
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genetic engineering deliberate modification of organism’s genetic  information by directly changing the sequence of  nucleic acids in its genome recombinant DNA technology procedures used to carry out genetic engineering 2 Some Terminology
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cloning  generation of a large number of genetically  identical DNA molecules biotechnology use of organisms to form useful products industrial microbiology use of microbes to manufacture important  compounds or the use of microbes as  products in their own right 3 More Terminology
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Key Developments in  Recombinant DNA Technology Restrictions enzymes Genetic cloning and  cDNA synthesis Southern blotting 4
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5 Figure 15.1
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Restriction Enzymes recognize and bind specific  sequences in DNA called   recognition sites cleave DNA at this site or a  defined distance from it   may produce sticky ends or  blunt ends in the DNA target hundreds are commercially  available 6 Figure 15.2
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7 Figure 15.3
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8 Table 15.2
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Jackson, Symons, and Berg (1972) generated first recombinant DNA molecules foreign DNA target can be recombined into plasmid  vector plasmid vectors replicate and maintain foreign  fragment vectors – carriers of foreign DNA 9 Genetic Cloning and cDNA  Synthesis
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synthesizes double stranded DNA from RNA  template (mRNA) used to construct complementary DNA (cDNA) independently discovered by Temin and Baltimore in  1970 10 cDNA Synthesis and Reverse  Transcriptase
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11 Figure 15.4
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12 Figure 15.5
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Edwin M. Southern (1975) developed for use in  detecting specific DNA fragments 3 step process
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