LAB 4 BIO FINAL

LAB 4 BIO FINAL - Harpreet Singh, Chris Sutera, Tony Tran,...

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Harpreet Singh, Chris Sutera, Tony Tran, Carlos November 30, 2011 Biology Lab Report Introduction For the experiment, we crossed two saccharomyces cerevisiae haploid strains Haploid strains appears during Meiosis I creating spores The spores become either “a” or “Alpha” differentiating the mating type of the yeast cells As the haploids develop, they can begin mating with other haploids. The haploids secrete pheromones which signify mating and later becoming shmoos (two yeast haploids combining). The shmoos creates space and undergoes meiosis I which forms a diploid zygotes As the zygotes undergoes essentially mitosis cell division leading to budding which creates diploid daughter cells Budding continues, and the zygote becomes a diploid colony The colony will repeat the cycle becoming two haploid strains once again Alpha 1 and a1are dominant and white. Alpha 2 and a2 are recessive and red The parents looked very lumpy. The two dominant strains were white and the two recessive were red Our predicted phenotype ratio was 3: 1 and our hypothesis was to compared our prediction and the actual yield Our hypothesis was that Alpha 1 and a1, Alpha 1 and a2, Alpha 2 and a1 would yield a white
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LAB 4 BIO FINAL - Harpreet Singh, Chris Sutera, Tony Tran,...

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