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Yeast Mating Weeks 7-90

Yeast Mating Weeks 7-90 - Yeast Mating I II III(Weeks 7-9...

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Yeast Mating I, II, III (Weeks 7-9) Names _________________________________________ _____________________________________ Section ____________ Date__________________ Lab TA ____________________________________ Yeast Mating Schedule Yeast Mating I: Week 7 Yeast Mating Prelab Steps 1: Complete hypothesis (in groups) Steps 2: Grow haploid yeast strains (a and alpha) on YED (in groups) Steps 3: Microscopic observation of haploid and mating yeast (in groups) Steps 4: Answer questions (in groups) Submit this document for grading of Step 4 questions (1.5%) Yeast Mating II: Week 8 Step 5: Observe colors of haploid strains (in groups) Step 6: Mate haploids (in groups) Yeast Mating III: Week 9 Step 7: Observe colors of diploid mated yeast (in groups) Step 8: Complete Questions (in groups) Submit this document for grading of Step 8 questions in class (1.5%) Group Final Yeast Mating Report: Due Week 11 (6%) Purpose : In Yeast Mating I-III, students will cross (mate) two haploid strains of Saccharomyces cerevisiae of opposite mating types (alpha and a) to create diploid yeast strains. The haploid strains are R and r with phenotypes of white and red, respectively. The diploid heterozygote is white. Goals: To understand haploid and diploid stages of the S. cerevisiae life-cycle. To understand alpha and a mating types To learn to culture and mate S. cerevisiae To observe various forms of S. cerevisiae during the life-cycle
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To predict the phenotypes of diploids that result from crosses between two different haploid strains To observe and discuss results of mating and compare to predictions Yeast Mating I (Week 7): Steps 1-4 Materials: Master plates of each of 4 strains (a1R, a2r, alpha1R, alpha2r) on YEAD sterile plates (1/group of 2-4 students) Sterile toothpicks
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