Exam4KEY - 1 Biochemistry I (CH 339K) Fall 2010 Unique...

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1 Biochemistry I (CH 339K) Fall 2010 Unique #51615 Professor Adrian Keatinge-Clay Exam #4 November 23, 2010 Do not begin work on this exam until you are instructed to begin. The core values of The University of Texas at Austin are learning, discovery, freedom, leadership, individual opportunity, and responsibility. Each member of the University is expected to uphold these values through integrity, honesty, trust, fairness, and respect toward peers and community. The work that you hand in will be considered to be your own original work prepared without assistance. I will uphold the honor code on this exam. Signed Name: KEY Printed Name: _________________________ EID: _______________
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2 1. Arsenate (AsO 4 ) is toxic to many species of anaerobic bacteria. The chemical properties of arsenate are very similar to inorganic phosphate; however, the mixed anhydride below is unstable due to its rapid hydrolysis in water. Propose how arsenate adversely affects glycolysis. (14 points) The arsenate ion can attack where P i normally would in the glyceraldehyde 3-phosphate dehydrogenase reaction. This attack forms an acyl arsenic derivative of 1,3- bisphosphoglycerate, which is rapidly hydrolyzed. The subsequent ATP generation step cannot occur. Therefore, no energy will be obtained from glycolysis in the presence of arsenate. 2. Consider phosphoglycerate kinase in the glycolytic pathway, which is known not to react through a phosphoenzyme intermediate. Draw the reaction mechanism showing all relevant parts of the molecules in atomic detail. (12 points) CH 2 CH 2 OPO 3 2- -O P O O - O C O -O P O -O O P O O - O Adenosine CH 2 CH 2 OPO 3 2- O - C O O P O O - O P O O - O Adenosine P O -O -O Mg 2+ Mg 2+ O As R O - O O - O acyl arsenate
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3 3. Triosephosphate isomerases contain a conserved 10-residue loop. If this flexible loop
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This note was uploaded on 02/06/2012 for the course BIOCHEMIST 339K taught by Professor Keating-clay during the Spring '11 term at University of Texas at Austin.

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Exam4KEY - 1 Biochemistry I (CH 339K) Fall 2010 Unique...

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