ExtraCreditHomework8KEY - H O Zn 2+ H O OH H O P O O O H A...

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Name: KEY EID: Extra Credit Homework #8 Complete questions below and hand in to a TA by the end of class, Friday, Nov. 4. 1. Glucose labeled with C-14 at the fourth carbon is fed to Saccharomyces cerivisiae (ale yeast). a) What fraction of the ethanol produced through fermentation will contain the C-14 label? None, 0% b) At which position is the C-14 located in the labeled ethanol? CO 2 is eliminated from pyruvate via decarboxylation, so it isn’t present on the ethanol at all. 2. a) Why are prokaryotic aldolases, but not animal aldolases, inactivated by ethylenediaminetetraacetate? EDTA chelates metal cations. Prokaryotic aldolases require Zn 2+ to stabilize negative charge formation, whereas eukaryotic aldolases use a Schiff-base-forming lysine residue. b) Draw the reaction mechanism for prokaryotic aldolase (you can skip the ring-opening and ring-closing reactions). H 2 C H H HO O H OH H CH 2 OPO 3 2- OPO 3 2- O Zn 2+ B H HO
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Unformatted text preview: H O Zn 2+ H O OH H O P O O O H A O P O O O O HO H G3P DHAP O P O O O 3. What is the reaction logic behind converting glucose 6-phosphate to fructose 6-phosphate in the preparatory phase of glycolysis? Moving the carbonyl allows for the formation of an electron sink in a position where fructose 1,6 bisphosphate can be split into two three-carbon units by aldolase. 4. In anaerobic organisms, how is the NAD + consumed in glycolysis regenerated? Lactate dehydrogenase regenerates NAD + by converting pyruvate into lactic acid. Alcohol dehydrogenase regenerates NAD + by converting acetaldehyde into ethanol. Each of these enzymes utilizes NADH as a reducing agent. 5. How many NADH and FADH 2 molecules are generated through the -oxidation of palmitic acid to acetyl-CoA? How many molecules of acetyl-CoA are generated? 7 NADH, 7 FADH 2 , and 8 acetyl-CoA are generated....
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This note was uploaded on 02/06/2012 for the course BIOCHEMIST 339K taught by Professor Keating-clay during the Spring '11 term at University of Texas at Austin.

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ExtraCreditHomework8KEY - H O Zn 2+ H O OH H O P O O O H A...

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