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Unformatted text preview: P E R S O N A L U S E O N L Y Vanadate affects proliferation of healing fibroblasts, cellular production of collagen, and expression of alpha-SMA in vitro Zhi-Tao Jiang ABCDEF , Zhao-Zhu Li ADEG , Da-Peng Jiang BDFG Department of Pediatric Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China Source of support: This study was supported by grants from the Natural Science Foundation of Heilongjiang (D200618) and National Natural Science Foundation of China (30901516) Summary Background: The medial collateral ligament of the knee is frequently injured in sports. The medial collateral ligament healing is slow. Vanadate is a transition element that has been shown to be a nonspecif- ic inhibitor of protein tyrosine phosphatases. It has notable effects on wound healing. This study sought to examine the effect of vanadate on proliferation, collagen type I, and a-smooth muscle actin (alpha-SMA) production in healing fibroblasts in rat (Sprague-Dawley). Material/Methods: Fibroblasts were obtained from a healing medial collateral ligament. Cell cultures were treated with different concentrations of vanadate (5, 10, 20 µmol/L). Healing fibroblasts without vanadate treatment were used control group. Fibroblast proliferation was measured using a 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Production of collagen type I in a supernatant culture was assayed by enzyme-linked immunosorbent assay (ELISA). Expression of alpha-SMA was assessed by Western blot. Results: At concentrations of 5 µmol/L, 10 µmol/L, 20 µmol/L of vanadate, fibroblast proliferation was significantly increased. Treatment with vanadate significantly increased the production of colla- gen type I and decreased a-SMA expression in healing fibroblasts in a dose-dependent manner. Conclusions: Our results showed that vanadate therapy improves ligament repair via increase in proliferation and collagen type I production, and a decrease in alpha-SMA expression in healing fibroblasts. The results provide a cellular and molecular basis for vanadate’s action as a therapeutic agent for enhancing medial collateral ligament healing and reducing scar formation. key words: vanadate • fibroblast proliferation • collagen • alpha-smooth muscle actin • medial collateral ligament • myofibroblast Full-text PDF: http://www.medscimonit.com/fulltxt.php?ICID=878493 Word count: 2554 Tables: — Figures: 3 References: 23 Author’s address: Zhao-Zhu Li, Department of Pediatric Surgery, Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Harbin, 150086, China, e-mail: [email protected] Authors’ Contribution: A Study Design B Data Collection C Statistical Analysis D Data Interpretation E Manuscript Preparation F Literature Search G Funds Collection Received: 2009.07.15 Accepted: 2009.09.28 Published: 2010.04.01 BR107 Basic Research WWW. M ED S CI M ONIT.COM © Med Sci Monit, 2010; 16(4): BR107-111 PMID: 20357710 BR Current Contents/Clinical Medicine • IF(2008)=1.514 • Index Medicus/MEDLINE • EMBASE/Excerpta Medica • Chemical Abstracts • Index CopernicusCurrent Contents/Clinical Medicine • IF(2008)=1....
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- Spring '10
- Collagen, Medial collateral ligament, Vanadate