BIOS E-1a final exam f10

BIOS E-1a final exam f10 - 1 Name: _ BIOLOGY E-1a:...

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1 Name: _____________________________________________________________ BIOLOGY E-1a: Introduction to Molecular and Cellular Biology Fall 2010 FINAL EXAM Teaching Fellow: ______________________________________________________ Be sure to write your name on the top of each of the pages of the exam. Write each answer only on the same page as the pertinent question. THE SPACE PROVIDED IS MEANT TO BE SUFFICIENT; BE BRIEF, BE CAREFUL, BE CONCISE! If absolutely necessary, use the back of that page to continue the answer. IF YOUR ANSWER CANNOT BE READ, IT CANNOT BE GRADED!!! Write out the word “True” or “False” for those types of questions, respectively. A codon table, periodic table, and table of amino acids are included at the end to aid in answering some of the questions. Remember, if you do not show all your work, you cannot get partial credit. The point values for each question are noted. Allocate your time wisely. Read carefully, think before you write, good luck, and do well! IF YOU WRITE IN PENCIL, YOU CANNOT ASK FOR A REGRADE! Page 2 (10 pts.) Page 3 (08 pts.) Page 4 (17 pts.) Page 5 (08 pts.) Page 6 (03 pts.) Page 7 (11 pts.) Page 8 (09 pts.) Page 9 (23 pts.) Page 10 (06 pts.) Page 11 (05 pts.) Total (100 pts.)
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2 (5 pts.) 1. In the 1920s, Griffith showed that there was a biochemical genetic material that could transform type R bacteria into type S. The Avery-MacLeod-McCarty experiment showed that the transforming genetic material was DNA. Briefly, DNA was purified from type S bacteria. Five conditions were tested: (1) type R bacteria only, (2) type R bacteria + DNA, (3) type R bacteria + DNA + DNase, (4) type R bacteria + DNA + RNase, (5) type R bacteria + DNA + protease. After allowing for enough time for type R cells to take up the type S DNA, the DNase, RNase, and protease enzymes were inactivated. Then an antibody was added to all the conditions that specifically recognizes type R cells that have not been transformed. The binding of the antibody causes the R cells to aggregate, allowing their removal by centrifuge. The remaining bacteria (if any) were plated. Predict the expected results for each condition by answering YES if there should be bacterial growth or NO if there should be very little or no growth on the plate. __________ Condition (1) type R only __________ Condition (2) type R + DNA __________ Condition (3) type R + DNA + DNase __________ Condition (4) type R + DNA + RNase __________ Condition (5) type R + DNA + protease (4 pts.) 2. You are investigating a new species of fly that has either orange eyes or blue eyes. A true-breeding orange-eyed male is crossed with a true-breeding blue-eyed female. The F 1 generation is all blue-eyed. However, in the F 2 generation, there are 1018 blue-eyed males, 882 orange-eyed males, 1900 blue-eyed females, and 0 orange- eyed females. True or false – this data allow you to conclude that: __________ the eye color gene is located on the sex chromosome
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This note was uploaded on 02/09/2012 for the course BIOLOGY 102 taught by Professor Anderson during the Spring '11 term at Harvard.

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BIOS E-1a final exam f10 - 1 Name: _ BIOLOGY E-1a:...

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