BIOS E-1a exam 02 120110

BIOS E-1a exam 02 120110 - 1 Name: _ BIOLOGY E-1a:...

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1 Name: _____________________________________________________________ BIOLOGY E-1a: Introduction to Molecular and Cellular Biology Fall 2010 EXAM 2 Teaching Fellow: ______________________________________________________ Be sure to write your name on the top of each of the pages of the exam. Write each answer only on the same page as the pertinent question. THE SPACE PROVIDED IS MEANT TO BE SUFFICIENT; BE BRIEF, BE CAREFUL, BE CONCISE! If absolutely necessary, use the back of that page to continue the answer. IF YOUR ANSWER CANNOT BE READ, IT CANNOT BE GRADED!!! Write out the word “True” or “False” for those types of questions, respectively. A codon table is included at the end to aid in answering some of the questions. Remember, if you do not show all your work, you cannot get partial credit. The point values for each question are noted. Allocate your time wisely. Read carefully, think before you write, good luck, and do well! IF YOU WRITE IN PENCIL, YOU CANNOT ASK FOR A REGRADE! Page 2 (03 pts.) Page 3 (03 pts.) Page 4 (30 pts.) Page 5 (05 pts.) Page 6 (14 pts.) Page 7 (09 pts.) Page 8 (11 pts.) Page 9 (13 pts.) Page 10 (12 pts.) Total (100 pts.)
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2 _____ (1 pt.) 1. Crossovers generally occur during: a) mitosis b) meiosis I c) meiosis II d) with equal likelihood in all three _____ (1 pt.) 2. Below is a cartoon depicting only human chromosome 2 (the other 22 chromosomes are not shown for simplicity but are behaving in a similar manner), aligning during metaphase of: a) mitosis b) meiosis I c) meiosis II d) not enough information is shown _____ (1 pt.) 3. In the cartoon below of a newly initiated replication bubble, identify the leading strand. Solid line represents the original DNA and the dashed line represents newly synthesized DNA/RNA (NOT Okazaki fragments). a) strand A b) strand B c) not enough information shown d) both A and B
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3 QUESTIONS 4-6: The Meselson-Stahl experiment showed that DNA replication is semi- conservative. Briefly, bacteria were grown in 15 N media (heavy) before being transferred to 14 N media (light). After the transfer, the bacteria were allowed to continue growing for 1, 2, or 3 more generations. The DNA was isolated after each generation and transferred to a CsCl gradient, and centrifuged in order to separate the DNA based on weight. (A strand of DNA synthesized while growing in 15 N will be heavy and a strand synthesized while in 14 N will be light. Heavy strands will migrate farther than light strands during centrifugation.) The DNA banding pattern was then visualized after centrifugation. _____ (1 pt.)
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BIOS E-1a exam 02 120110 - 1 Name: _ BIOLOGY E-1a:...

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