Enzymes and Ant Collection E1a 2011

Enzymes and Ant Collection E1a 2011 - Enzymes and Ant...

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Unformatted text preview: Enzymes and Ant Collection for PCR lab Friday, September 9, 2011 Collect one carpenter ant if you are given an orange tube of ethanol. Store cold and bring in next lab. Friday, September 9, 2011 Collect one pavement ant if you are given a yellow tube of ethanol. Store cold and bring in next lab. Friday, September 9, 2011 Enzymes • Biomolecules that catalyze specific chemical reactions. • Most enzymes are proteins. • Some are RNA or Protein & RNA. • Shape of the enzyme and chemical charge of amino acid residues in the active site cause them to have a specific catalytic activity on very specific substrates. • Activity can be changed by altering pH, temperature or inhibitor molecules. Friday, September 9, 2011 Succinic Dehydrogenase Inner Mitochondrial Membrane Friday, September 9, 2011 Succinic Dehydrogenase Activity COOH COOH SUCCINIC D EHYDROGENASE CH 2 CH CH 2 CH FAD COOH Succinic Acid ! Friday, September 9, 2011 FADH 2 (MB) (MB-H2 ) COOH Fumaric Acid Competitive inhibitors resemble substrates in shape and charge COOH CH 2 COOH Malonic Acid Succinic Acid Friday, September 9, 2011 ! α-Amylase Green=Chloride Yellow=Calcium Friday, September 9, 2011 Starch Structure is hydrolyzed by α-Amylase at α-1,4-glycosidic bonds α-1,4 linkages Friday, September 9, 2011 pH effects Enzyme Activity Each enzyme shows a peak of activity at its optimum pH Friday, September 9, 2011 Scientific Graphing - XY Scatter Plots Y-axis plots measured results. Figure # and Title (above or below graph) Axis Labels and Units Friday, September 9, 2011 Data Point Trendline X-axis plots the variable you set as experimental condition. Scientific Hypotheses • Hypothesis - educated guess or question into how a natural phenomenon works. • Testable - can be used as the basis of an experiment. Data from the experiment may support the hypothesis or refute it. • Hypothesis usually is stated as a sentence. • Example - “Hypothesis: Glucose acts as an allosteric inhibitor of Glycogen Phosphorylase activity.” • Write up: Make your hypothesis obvious so that your TF will grade it easily so that you will get full credit! Friday, September 9, 2011 ...
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Enzymes and Ant Collection E1a 2011 - Enzymes and Ant...

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