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BIOS 2011 Lab 2 Photosynthesis

22 exercise 2 photosynthesis protocol 3 data

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Unformatted text preview: ait for this to finish. Then insert your blank cuvette into the spectrometer, again making sure cuvette is aligned correctly. Click Finish Calibration. The spectrometer will zero itself at 600nm, so that the chloroplasts’ absorbance at this wavelength is ignored by the spectrometer. 3. Label a sample cuvette with the number 30 at the top, just by the triangle. Being very sure that you shield it from light, prepare an experimental cuvette. Use the same volume of chloroplasts as in the blank cuvette above. µl chloroplasts (15 mg chlorophyll) 300 µl of DCPIP solution 2.6 ml of phosphate buffer Mix until homogenous with a 1000µL pipetter. Immediately read the solution's absorbance at 600 nm. This is your time=0 reading. 4. Place the cuvette in a marked location 30 cm from the 100W light bulb with label facing away from th...
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