BIOS 2011 Lab 2 Photosynthesis

In differential centrifugation two or more

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Unformatted text preview: sport chain. More electron transport results in the formation of a greater pH gradient across the thylakoid membrane; this leads to more ATP production (at the expense of NADPH production). In this way, the energy captured by the pigments is diverted to produce more ATP. Exercise 2 Photosynthesis PROTOCOL 1: ISOLATION OF SPINACH CHLOROPLASTS Note: This procedure will be done for you in advance by the TFs. The technique of cell fractionation is used to isolate chloroplasts from plant tissue. Spinacia oleracea or spinach leaves are homogenized in a blender with phosphate buffer to break up the cells. The resulting homogenate is strained through cheesecloth to remove large debris. Next, differential centrifugation is employed to separate the chloroplasts from other cell debris. During centrifugation, denser objects will migrate to the bottom of the centrifuge tube faster...
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This note was uploaded on 02/09/2012 for the course BIOLOGY 102 taught by Professor Anderson during the Spring '11 term at Harvard.

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