BIOS 2011 Lab 2 Photosynthesis

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Unformatted text preview: than less dense objects. In differential centrifugation, two or more centrifugation speeds are used to separate parts of the cell based on their different densities. First, the homogenate is centrifuged at a low speed to separate large and dense cell debris such as nuclei, cell walls, and unbroken cells. This unwanted debris that sediments at the bottom of the test tube is called the pellet, and is discarded. The remaining liquid, called the supernatant, contains chloroplasts. The chloroplast ­containing supernatant is subjected to a second centrifugation at much higher speed. This high speed spin causes the chloroplasts to sediment into a pellet. This second pellet is resuspended in phosphate buffer and the highly purified chloroplasts will be used for the three experiments in this lab. PROTOCOL 2: SPECTRAL ANALYSIS OF CHLOROPLAST PIGMENTS You will be using a Vernier USB scanning spectrophotometer...
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