Leture+10+Cell+Growth+and+Imaging

Leture+10+Cell+Growth+and+Imaging - Basic Microscopy &...

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Basic Microscopy & Imaging Methods r Measurement of Cellular for Measurement of Cellular Properties Lecturer: Prabhas Moghe Lab Assistant: Leora Nusblat March 22, 2011 125:315 Biomedical Measurements and Analysis Lab
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Overview of the Lab Exercises icroscopy and Cellular Growth/Imaging Microscopy and Cellular Growth/Imaging Purpose The purpose of this laboratory exercise is to develop an understanding of the measurements of cellular property via biomedical imaging and microscopy techniques. After completing this boratory experiment you should be able to: laboratory experiment, you should be able to: 1. Assay cell population viability through the use of a live/dead staining (calcein/ethidium homodimer stain). 2. Obtain total cell population and percentage dead cells and quantify on the parameter basis of color and cell size/diameter using Image J software. 3. Quantify, compare and relate different morphological cell configurations to cellular differentiation. Learn how to : recall images obtained on a computer-interfaced optical microscope process images and quantify results identify cells via fluorescence labeling of the cytoskeleton show cells are viable via fluorescence labeling of DNA visualize cell borders quantify cell morphology (phenotyping)
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General Resources rimer in Microscopy by Olympus: ttp://www olympusmicro com/primer/opticalmicroscopy html Primer in Microscopy by Olympus: http://www.olympusmicro.com/primer/opticalmicroscopy.html Molecular Expressions A comprehensive resource maintained by Florida State University. Includes a Microscopy Primer with tutorials on optical microscopy, digital imaging: http://micro.magnet.fsu.edu/primer/ microscopy.info An information source for microscopy and microanalysis including a hyperlinked glossary and links to professional societies and educational resources. http://www.mwrn.com World Wide Web Virtual Library: Microscopy: http://www.ou.edu/research/electron/www-vl/long.shtml Vast set of links covering all aspects of light, electron, and other types of microscopy, including extensive lists of imaging and microscopy labs, image galleries, and educational sites. Live Cell Imaging Applications in Confocal Microscopy: A lecture by Dr. J. Paul Robinson at Purdue Univ. http://www.cyto.purdue.edu/flowcyt/educate/confocal/524Lec11/sld001.htm NIH Office of Extramural Research: Links to Bioimaging Sites http://grants2.nih.gov/grants/bioimaging/links.htm Standard Microscopy Terminology: http://resolution.umn.edu/glossary/FrameGloss.html A comprehensive glossary of microscopy terms made available by the University of Minnesota Characterization Facility. Microscopy/Imaging Resources on WWW: http://swehsc.pharmacy.arizona.edu/exppath/micro/index.html A very well-organized, extensive collection of educational and informational resources, including links to numerous online image galleries, microscopy sites, and an introduction to digital imaging. * Science Magazine, April 2003, Special Issue on Biological Imaging
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Leture+10+Cell+Growth+and+Imaging - Basic Microscopy &...

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