Problem set 2 key

Problem set 2 key - BIOL/CHEM 3361 Biochemistry I Spring...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIOL/CHEM 3361 Biochemistry I Due: Tuesday, March 1 at the start of class Spring 2011 No late Problem Sets will be accepted. You may turn them in early, e.g. at lecture on Thursday Feb. 24, to avoid last minute emergencies. PROBLEM SET 2 For full credit, show all steps to the solutions of the following problems: 1. a. An alpha helix of exactly 21 amino acids is needed to span the plasma membrane of cells. Based on the dimensions of an alpha-helix, calculate the width of the membrane? b. How many amino acids would be needed in a beta strand to span the membrane? 2. For a Michaelis enzyme reaction, k 1 = 2.5 x 10 9 M -1 s -1 , k -1 = 5.0 x 10 3 s -1 , and k 2 = 5.0 x 10 4 s -1 . a. Calculate K S and K m for this reaction. b. Does substrate binding approach equilibrium or only achieve steady state, i.e. does the enzyme follow rapid equilibrium or only steady state kinetics? 3. The following table shows the kinetic analysis of a Michaelis enzyme in the presence of two different inhibitors, A and B, one a transition-state analog and the other NEM (N- ethylmaleimide). a. Make a fully labeled Lineweaver-Burk plot of the data and use it to deduce which inhibitor is A and which is B. Explain the basis of your assignment. b. Using the plot, determine the K m of the enzyme and the K I of the transition-state analog, given that it was present at 5 x 10 -4 M.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

Problem set 2 key - BIOL/CHEM 3361 Biochemistry I Spring...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online