CHEM3472LAB-Fluorescence-S2010 - Chemistry 3472...

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Chemistry 3472 Determination of quinine in tonic water using fluorescence spectroscopy Purpose: To determine the concentration of quinine in tonic water by developing an analytical method based on fluorescence. Underlying Chemistry Quinine undergoes protonation and deprotonation. There are potentially three species: H 2 Q, HQ - , and Q 2- . Only HQ - is fluorescent, and it is the dominant species in 0.05 M H 2 SO 4 . Hence, all runs must me made in 0.05 M H 2 SO 4 . Apparatus: Fluorimeter Quartz fluorimeter cuvette Standard analytical laboratory glassware Chemicals: 0.05 M H 2 SO 4 .: Concentrated sulfuric acid is about 18 M. First prepare 60 mL of 1 M M H 2 SO 4 ; then dilute this solution to prepare 0.05 M H 2 SO 4 . Quinine stock solution (100.0 ug/mL), 120.7 mg quinine sulfate dihydrate or 100.0 mg quinine, transfer to a 100 mL volumetric flask, and dilute to the mark with 0.05 M H 2 SO 4 . (prepare fresh daily; decomposes in light). Your will need to dilute this solution by a factor of 10. Pipet 10 mL into a 100 mL volumetric flask and dilute to the mark with 0.05 M H 2 SO 4 .. Unknown: Tonic drink containing quinine. You may chose to measure one or two different varieties. Pipet 5.00 mL of tonic water into a 250 mL volumetric flask, dilute to
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