BIOLAB Study Guide

BIOLAB Study Guide - Final Exam Study Guide Know common...

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Final Exam Study Guide Know common laboratory safety procedures. Pg viii o Chapter 1: o Know the names of pipettes. Know how to read pipettes. Mechanical : Set the desired volume on the knob, to draw liquid in, put the pipette in the liquid and push the plunger to the first stop. To eject the liquid, push the plunger to the second stop. Volumetric : (has a bulb). Draw the fluid to above the ring at the top of the bulb, then release slowly until the bottom of the meniscus is exactly at the ring. Then allow the liquid to drain out in a second container Mohr (Measuring ): meniscus must be at the collaboration mark at the beginning and end of a transfer Serologica l : have to subtract the desired volume from the total volume it can hold since the “0” mark is at the top of the pipette Pi pumps : always use them when drawing liquid in. o Be able to blank a spectrophotometer and figure out the concentration of a given solution. Left knob turns it on. Adjust to proper wavelength. (knob on the top to the right). Turn left hand knob to “0” on transmittance. Put in the “blank”, after wiping it with a kimwipe, line up the marking on the tube with the white mark on the spec. Adjust the transmittance to 100% (the right hand knob). Remove the blank and immediately put in the solution. Don’t change any instrument settings! Change mode to absorbance. Record the value. Chapter 2: o Properties of enzymes: e.g. – Activation energy, active site, induced-fit model, specificity A catalyst is required for most chemical reactions to occur. It reduces the activation energy and speeds up the reaction without being consumed in the reaction. Activation energy : energy required to produce an activated complex needed for a reaction to occur Enzymes : highly specific organic catalysts that make the chemical reactions that are a part of life possible. Active site : substrate attracted to this site, and reacts chemically with the amino acids that are there, causing it to change, resulting in the formation of the activated complex. Then the substrate is converted into the product. Induced fit model : Substrate attracted to active site, changing the shape of the enzyme, reacts with the enzyme, forms into the product, then the enzyme changes back, releasing the product and able to start a new reaction with other substrates.
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BIOLAB Study Guide - Final Exam Study Guide Know common...

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