Lab Report

Lab Report - Kendi Murungi Darin Ellair Section 6 November...

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Kendi Murungi Darin Ellair Section 6 November 15, 2009 Alexandra Arceneaux Lab Report Determining the Relative Osmotic Concentration of Potato Tuber Cells Abstract: The reasoning behind this experiment was to present a concept relative to the movement of materials across a cell membrane. To determine this, the molarity of the potato tuber cell must to be established. In order for the molarity to be determined, the potato tuber cells were submerged in various concentrations of an extracellular solution (sucrose) and dH 2 O for 60 minutes, and then extracted from the solution. The initial mass (which was already recorded) was divided by the change in mass to yield the percent changes in mass, which was then measured and recorded. There was a downward trend of the percent change in mass until it crossed the x- axis at 0.2M, where from then on there was an increase in the magnitude of the percent change in mass. Where there was no change in mass, the solution was considered isotonic to the potato and therefore indicated the relative osmotic concentration of the potato to be .3 M. Introduction: Membranes allow cells and organisms to control their internal chemical environment. The regulation of the movement of different substances can result in the control of numerous metabolic pathways by altering the accessibility of materials. Characteristically, biological membranes are comprised of phospholipid bilayers that also contain proteins. Some biological membranes are selectively permeable which results in select materials crossing the membrane while others cannot. The ability of molecule to cross the membranes has to do with many factors ranging from size, solubility, and charge. Hydrophobic molecules are lipid soluble and hydrophilic molecules are water soluble. Water itself is arguably the single most important molecule. Osmosis, the movement of water across biological membranes, is a
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This note was uploaded on 02/09/2012 for the course BIO 1201 taught by Professor Sibenaller during the Fall '10 term at LSU.

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Lab Report - Kendi Murungi Darin Ellair Section 6 November...

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