homework (5 out of 9)

homework (5 out of 9) - 1) The SNARE hypothesis is an...

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1) The SNARE hypothesis is an attempt to explain how the various vesicles in the cytoplasm, which are trafficking between numerous compartments, TARGET to and DOCK with appropriate targets. What additional function for the SNARE complex are the authors proposing to test in this paper? What is the overall strategy for testing their hypothesis? The additional function for the SNARE complex which the author is testing for is if the SNARE complex formation itself is sufficient enough to drive membrane fusion + contribute to targeting appropriate locations. The overall strategy for testing these hypotheses is to create a synthetic lipid vesicle with only v- SNARE or t-SNARE proteins, this will allow for sufficient amounts of observational data to be collected via an Assay fusion of these vesicles using BoNTD. 2) What is the source of the SNARE proteins used in these experiments? The souce of SNARE proteins used in these experiments were Recombinant SNARES, such as the vSNARE- synaptobrevin 2/ VAMP2, as well as the tSANRES – SNAP25 and syntaxin. 3) Briefly describe how the artificial lipid vesicles are created. Do all the proteins get
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homework (5 out of 9) - 1) The SNARE hypothesis is an...

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