practice_exam_1 - BMB/MICRB 460 NAME: Practice Exam 1 /...

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BMB/MICRB 460 NAME: Practice Exam 1 / TEST FORM A PLOT A PLOT B TEST DIRECTIONS: Choose a single answer for each multiple-choice question and RECORD it on the line to the left of the question. Write your answer to the short answer questions LEGIBLY in the space provided There are 25 questions, and each question is worth 4 points (unless noted). I will post the exam, the answers and your scores as soon as all students have taken the exam and grading is complete. 1. Hydropathy PLOT A was generated using the sequence of a protein that could not be extracted from the membrane pellet of lysed cells with high salt buffer but was extracted by TritonX-100. What hypothesis would you make about the nature and structure of the region of the protein that associates with the membrane? 2. PLOT B was generated using the sequence of a second protein with similar extraction properties. What hypothesis would you make about the nature and structure of this protein?
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BMB/MICRB 460 NAME: Practice Exam 1 / TEST FORM A A B A C D squirrel chip- munk 3. You plan to repeat the experiments of Frye and Edidin using some new cell lines: a squirrel-derived cell line and a chipmunk-derived cell line. You have as many animals available to you as you need of each of the following species for antibody production– rabbits, hamsters, and squirrels. A.Which animal will you inject with the squirrel cell line to effectively produce antibodies to many plasma membrane proteins? B. Which animal will you inject with the chipmunk cell line to effectively produce antibodies to many plasma membrane proteins? C. Choose an animal for production of a secondary antibody to the primary antibody produced in “A”. D. Choose an animal for production of a secondary antibody to the primary antibody produced in “B”. 4. Your experimental scheme is sketched below. At which step would you add the antibodies? a. add them to the individual cell lines prior to the fusion reaction – step A b. add them at the same time as the fusogen was added – step B c. add them as soon as the fusion reaction was complete -step C d. add them after the appropriate incubation time at 37 degrees – step D
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BMB/MICRB 460 NAME: Practice Exam 1 / TEST FORM A After repeating the Frye and Edidin experiments with your cell lines, you get results shown in the table below. Use this table to answer questions the next two questions. 5. Which antigens were diffusing fastest according to the data in the table? a. The antigens on the surface of the squirrel cells (S) b. The antigens on the surface of the chipmunk cells (C) c. They each moved at similar speeds d. There was no diffusion in this assay 6. How efficient was the fusion reaction that you performed? a.
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practice_exam_1 - BMB/MICRB 460 NAME: Practice Exam 1 /...

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