Chapter 14 DNA The Genetic Material 2011

Chapter 14 DNA The Genetic Material 2011 - Chapter 14 DNA...

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Chapter 14 DNA: The Genetic Material Learning Objectives: 1. Review of DNA Structure 2. Evidence for DNA as the genetic material 3. Models of DNA Replication 4. DNA replication in E. coli 5. Repair of DNA
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Review of DNA Structure 1. Model developed by Watson and Crick, Rosalind Franklin, and Erwin Chargaff 2. DNA is a double helix with a 2 nm diameter 3. The two helices of the double helix are composed of deoxyribose sugars alternating with phosphates covalently bonded by phosphodiester bonds 4. The two helices are anti-parallel or have opposite polarity. Each helix has a 5’ end (phosphate) and a 3’ end (-OH). 5. The two helices are held together by Hydrogen bonds of the two nitrogen bases.
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6. The nitrogen bases are either purines with 2 C-N rings (Adenine and Guanine) or pyrimidines with a single C-N ring (Thymine and Cytosine) 7. In the double helix a purine nitrogen base pairs with a pyrimidine nitrogen base via Hydrogen bonds.
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