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In class example-known parameters

In class example-known parameters - b At an enzyme...

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The enzyme, fumarase, has the following kinetics constants: Fumarase k 1 E P k ES + → 2 E+S k -1 where k 1 =10 9 M -1 s -1 , k -1 =4.4X10 4 s -1 , k 2 = 10 3 s -1 a. What is the value of the Michaelis constant for this enzyme? What is the Km in BH approach?
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Unformatted text preview: b. At an enzyme concentration of 10-6 M, what will be the initial rate of product formation at a substrate concentration of 10-3 M? Calculate them using the two approaches....
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