lecture notes-bioreactor analysis and operation-3-other consideration-lecture notes

Lecture notes-bioreactor analysis and operation-3-other consideration-lecture notes

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Immobilized Cell System Immobilized cells Provide high cell concentration Reuse cell Elimination of washout problem at high dilution rate and cell recovery May provide favorable microenvironmental conditions May prove genetic stability Protect against shear damage Can perform multi-step biosynthesis reactions that are not practical purified immobilized enzyme preparation. Disadvantages: Diffusional limitation are important. Growth and gas evalution may lead to significant mechanical disruption of the immobilizing matrix.
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Immobilized Cell System Immobilized methods: - Active immobilization of cells Entrapment and Binding similar to immobilized enzyme except covalently binding (toxic agents, e.g. glutaraldehyde) - Passive immobilization of cells: biological film. Multi-layer growth of cells on solid surface. e.g. common in biological waste water treatment and mold fermentation thick biofilm: high concentration but diffusion problem Bioreactor consideration: - low hydrodynamic shear: packed-column, fluidized-bed or airlift reactors.
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This note was uploaded on 02/13/2012 for the course CHM 3210 taught by Professor Kennell during the Fall '11 term at University of Florida.

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Lecture notes-bioreactor analysis and operation-3-other consideration-lecture notes

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