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lecture notes-bioreactor analysis and operation-Summary

lecture notes-bioreactor analysis and operation-Summary -...

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Summary of Bioreactor Design and Operation Modified batch and continuous reactors - Chemostat with cell recycle Keep high cell mass concentration in the reactor. Production of biomass and low-value product - Fed-batch Maintain low substrate concentration Secondary metabolites, prevent catabolite repression - Multi-stage chemostat reactor Separate cell growth and product formation Secondary metabolites, genetically modified cell culture
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Summary of Bioreactor Design and Operation Modified batch and continuous reactors - Chemostat with cell recycle ( q p =0 , k d ≈0, X 0 =0, Monod equation is applied ): ] ) 1 ( ) 1 ( 0 [ )] 1 ( 1 [ X1 ) 0 ( D 1 X ) 1 ( ) 1 ( )] 1 ( 1 [ 0, dS/dt state, steady At / / C D m C D s K S C Y S S Y g C D m C D s K S S s K S m g D C net g M S X M S X α α μ α α α μ α α μ α α μ μ α μ μ - + - - + - - + = - = - + - - + = + = - + = = =
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Summary of Bioreactor Design and Operation Modified batch and continuous reactors - Fed-batch ( q p =0 , k d ≈0, Monod equation is applied ): t Dt V X q V V V Ft X q t S FY t X t X V t X X D m D s K S S Y X S s K S m D V F net g Ft m p m p M S X M S X m ) 2 0 V ( 0 t P t P P constant, is q ), 2 0 V ( t P t P 0 , / , 0 X 0, dX/dt state, steady quasi at , 0 V V 0 p 0 / 0 / + + = = + + = + = = - = = = + = = = = = + = μ μ μ μ reactor in the mass cell initial
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Unformatted text preview: • Modified batch and continuous reactors- Multi-stage chemostat reactor ) 1 ( ) , ( , ) 1 (--= =--= n X n X n D n X net n S x X n x r n X n X n D n X net μ ) 1 ( 1 ) , ( , ) 1 ( ) , ( , /--=--= n S n S n D Y n S x X n s r n P n P n D n S x X n p r S X Summary of Bioreactor Design and Operation • Scale-up/down- Geometric similarity- Dynamics similarity • Sterilization- Probability of unsuccessful sterilization 1- P (t)= 1-[1-e-k d t ] N 0 - Deactivation of components in media C=C e-kdt • Immobilized cell systems Advantages and disadvantages • Operation consideration agitation and aeration, determination of volumetric mass transfer coefficient k L a, heat removal, foam, etc. Summary of Bioreactor Design and Operation...
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